##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename Trimmomatic on N_r2 _R2 paired_.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10602766 Total Bases 1 Gbp Sequences flagged as poor quality 0 Sequence length 27-98 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.24859522505731 37.0 37.0 37.0 32.0 37.0 2 34.746840305633455 37.0 37.0 37.0 27.0 37.0 3 38.837276423906744 41.0 37.0 41.0 37.0 41.0 4 37.852960821732744 41.0 37.0 41.0 32.0 41.0 5 38.80500503359218 41.0 41.0 41.0 37.0 41.0 6 39.368580047885615 41.0 41.0 41.0 37.0 41.0 7 39.57482028746084 41.0 41.0 41.0 37.0 41.0 8 39.62116093102498 41.0 41.0 41.0 37.0 41.0 9 39.63679666230491 41.0 41.0 41.0 37.0 41.0 10-11 39.71657881537705 41.0 41.0 41.0 37.0 41.0 12-13 39.71308963151691 41.0 41.0 41.0 37.0 41.0 14-15 39.645244410750934 41.0 41.0 41.0 37.0 41.0 16-17 39.7236163657672 41.0 41.0 41.0 37.0 41.0 18-19 39.726242708742234 41.0 41.0 41.0 37.0 41.0 20-21 39.705963707960734 41.0 41.0 41.0 37.0 41.0 22-23 39.70966679826755 41.0 41.0 41.0 37.0 41.0 24-25 39.68109283935909 41.0 41.0 41.0 37.0 41.0 26-27 39.6630093977364 41.0 41.0 41.0 37.0 41.0 28-29 39.670340929612905 41.0 41.0 41.0 37.0 41.0 30-31 39.67680644405553 41.0 41.0 41.0 37.0 41.0 32-33 39.6261185762505 41.0 41.0 41.0 37.0 41.0 34-35 39.61559413222844 41.0 41.0 41.0 37.0 41.0 36-37 39.62938331305287 41.0 41.0 41.0 37.0 41.0 38-39 39.63922715776148 41.0 41.0 41.0 37.0 41.0 40-41 39.43874792400733 41.0 41.0 41.0 37.0 41.0 42-43 39.30952544023891 41.0 41.0 41.0 37.0 41.0 44-45 39.26693047013231 41.0 41.0 41.0 37.0 41.0 46-47 39.22505640818103 41.0 41.0 41.0 37.0 41.0 48-49 39.23960215608194 41.0 41.0 41.0 37.0 41.0 50-51 39.23774493345199 41.0 41.0 41.0 37.0 41.0 52-53 39.20797487895506 41.0 41.0 41.0 37.0 41.0 54-55 39.18775200993029 41.0 41.0 41.0 37.0 41.0 56-57 39.15794987994058 41.0 41.0 41.0 37.0 41.0 58-59 39.1329511877758 41.0 41.0 41.0 37.0 41.0 60-61 39.12419520396617 41.0 41.0 41.0 37.0 41.0 62-63 39.15853481232671 41.0 41.0 41.0 37.0 41.0 64-65 39.09587088172705 41.0 41.0 41.0 37.0 41.0 66-67 38.942055465383795 41.0 41.0 41.0 34.5 41.0 68-69 39.0172225694155 41.0 41.0 41.0 37.0 41.0 70-71 39.07437641447987 41.0 41.0 41.0 37.0 41.0 72-73 39.03519877059063 41.0 41.0 41.0 37.0 41.0 74-75 38.1048675452154 41.0 37.0 41.0 32.0 41.0 76-77 38.91162656947379 41.0 41.0 41.0 34.5 41.0 78-79 39.111415397605235 41.0 41.0 41.0 37.0 41.0 80-81 39.12284559464611 41.0 41.0 41.0 37.0 41.0 82-83 39.061908955590056 41.0 41.0 41.0 37.0 41.0 84-85 39.0320901348299 41.0 41.0 41.0 37.0 41.0 86-87 39.06354925815639 41.0 41.0 41.0 37.0 41.0 88-89 39.062268211887414 41.0 41.0 41.0 37.0 41.0 90-91 38.96917480846631 41.0 41.0 41.0 37.0 41.0 92-93 38.93496377830438 41.0 41.0 41.0 37.0 41.0 94-95 38.86819147452549 41.0 41.0 41.0 37.0 41.0 96-97 38.78846634882656 41.0 41.0 41.0 34.5 41.0 98 37.25129964343408 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3207938008879765 1101 2 -0.35568316500863517 1101 3 -0.4592689197514588 1101 4 -0.42304104903283957 1101 5 -0.33208147249136744 1101 6 -0.5080763151090437 1101 7 -0.554314957867895 1101 8 -0.5311243910513639 1101 9 -0.5073700436367972 1101 10-11 -0.7121603793154279 1101 12-13 -0.4351457171477122 1101 14-15 -0.5637317049181192 1101 16-17 -0.5837943799307155 1101 18-19 -0.7278775554398393 1101 20-21 -0.5677061780957118 1101 22-23 -0.7764642926480576 1101 24-25 -0.5739718949273751 1101 26-27 -0.6326352389412833 1101 28-29 -0.8223639076007387 1101 30-31 -0.811542757869482 1101 32-33 -0.7567131835059442 1101 34-35 -0.6955474047132384 1101 36-37 -0.8280980898178072 1101 38-39 -0.7118186010386296 1101 40-41 -0.8988818302920336 1101 42-43 -0.8355320319114483 1101 44-45 -0.8731786241388377 1101 46-47 -0.8970208228708856 1101 48-49 -1.0129048858825627 1101 50-51 -0.9267841785973729 1101 52-53 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2226 30-31 0.16389983922436357 2226 32-33 0.23350996336819207 2226 34-35 0.22940215771651395 2226 36-37 0.27567192503099136 2226 38-39 0.30012920829658896 2226 40-41 0.2865092805354834 2226 42-43 0.30728404242486107 2226 44-45 0.3091479392609884 2226 46-47 0.38392826250179724 2226 48-49 0.4008306074031722 2226 50-51 0.34949683104564855 2226 52-53 0.33447098743183545 2226 54-55 0.3202269439164027 2226 56-57 0.4038655850296635 2226 58-59 0.42019893593280244 2226 60-61 0.3376844616375081 2226 62-63 0.3979164460871516 2226 64-65 0.44540838380601855 2226 66-67 0.5848640596717232 2226 68-69 0.4565644319067914 2226 70-71 0.33582742723724834 2226 72-73 0.3575212618222494 2226 74-75 0.5338501242370199 2226 76-77 -1.5563781571726878 2226 78-79 -0.9772796813894189 2226 80-81 0.14907868284146275 2226 82-83 0.3733793640545713 2226 84-85 0.3715784641775528 2226 86-87 0.38997125662912424 2226 88-89 0.31618611023645116 2226 90-91 0.3700420533027682 2226 92-93 0.27364104167384795 2226 94-95 0.31373729711219767 2226 96-97 0.4456571830326084 2226 98 0.5489789464816894 2227 1 0.012936902622499247 2227 2 -0.06563703516457764 2227 3 0.2040880159104006 2227 4 -0.021869773017307637 2227 5 0.054054760056999385 2227 6 0.17265431500224793 2227 7 0.22680255112133807 2227 8 0.17394250155220448 2227 9 0.2351917064977087 2227 10-11 0.2437809043935104 2227 12-13 0.22447491731102076 2227 14-15 0.30858715338974463 2227 16-17 0.28905771137069536 2227 18-19 0.29525055617656193 2227 20-21 0.2214603329252398 2227 22-23 0.14509570147654216 2227 24-25 0.2189375405186169 2227 26-27 0.2613344632717727 2227 28-29 0.2716429112636334 2227 30-31 0.24945932854954123 2227 32-33 0.23039870921091676 2227 34-35 0.22827566914232733 2227 36-37 0.28849243785150946 2227 38-39 0.2305551282622602 2227 40-41 0.25679143910215885 2227 42-43 0.3662369444740037 2227 44-45 0.4108537648161885 2227 46-47 0.42619840519034824 2227 48-49 0.38071474000697236 2227 50-51 0.27970818175909073 2227 52-53 0.35501599333247924 2227 54-55 0.3670566832147557 2227 56-57 0.38573448321655235 2227 58-59 0.47834720328609137 2227 60-61 0.4980213353634966 2227 62-63 0.43042368779941853 2227 64-65 0.42180576606114073 2227 66-67 0.6388818689196611 2227 68-69 0.5858960486861022 2227 70-71 0.43055975209509967 2227 72-73 0.4569204679160137 2227 74-75 0.6993334771624617 2227 76-77 0.560684196375739 2227 78-79 0.4780289070715895 2227 80-81 0.39149521739856397 2227 82-83 0.37983249650370965 2227 84-85 0.48478964740851893 2227 86-87 0.41297865878659223 2227 88-89 0.3505647256910933 2227 90-91 0.360359082323086 2227 92-93 0.31450498637425284 2227 94-95 0.43456069350033033 2227 96-97 0.47545796221656644 2227 98 0.5170305723969193 2228 1 0.059818336555380824 2228 2 -0.18514740176474476 2228 3 -6.240208296760827 2228 4 -3.0821859688894975 2228 5 -1.02485624742679 2228 6 -0.2612766147983834 2228 7 0.15442990048828165 2228 8 0.14489011695255982 2228 9 0.12201372974292468 2228 10-11 0.2209579261365846 2228 12-13 0.19047137156224636 2228 14-15 0.2326879873045371 2228 16-17 0.2308849357291436 2228 18-19 0.24718663791154682 2228 20-21 0.2025738564526307 2228 22-23 0.1889164397408436 2228 24-25 0.19983268058997794 2228 26-27 0.1813501226072063 2228 28-29 0.2822343059978536 2228 30-31 0.2315857260697527 2228 32-33 0.24845441963396553 2228 34-35 0.28199296782256766 2228 36-37 -0.32680396392601097 2228 38-39 -0.11917157186227456 2228 40-41 0.24469026888819911 2228 42-43 -0.23110948959440236 2228 44-45 0.26843163827908256 2228 46-47 0.39193265321844706 2228 48-49 0.35851526234189635 2228 50-51 0.37161474967196284 2228 52-53 0.4319038497353418 2228 54-55 0.43959886303385787 2228 56-57 0.4124210876191867 2228 58-59 0.41229509412065823 2228 60-61 0.4711432092380008 2228 62-63 0.43202516647696143 2228 64-65 0.4628705391947321 2228 66-67 0.5329360283413749 2228 68-69 -4.322149502970312 2228 70-71 -0.25455305338814327 2228 72-73 0.3445814536913048 2228 74-75 0.6357063071728035 2228 76-77 0.5039561392283929 2228 78-79 0.3728064118594716 2228 80-81 0.39100417465996884 2228 82-83 -3.619659590447995 2228 84-85 -1.8792522909574032 2228 86-87 -0.9129691373008413 2228 88-89 0.1210006309677567 2228 90-91 0.25005877354371364 2228 92-93 0.34090624966267313 2228 94-95 0.361301305931093 2228 96-97 0.4057314366718501 2228 98 0.5526519419602494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 9.0 14 80.0 15 508.0 16 2003.0 17 5042.0 18 8525.0 19 11346.0 20 14220.0 21 17536.0 22 21371.0 23 25348.0 24 29358.0 25 34078.0 26 39382.0 27 45767.0 28 52672.0 29 61701.0 30 72649.0 31 85681.0 32 101997.0 33 123897.0 34 152417.0 35 193851.0 36 256702.0 37 361696.0 38 593140.0 39 1384803.0 40 6906986.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.473884078928084 24.80059448638214 25.771765593996886 25.953755840692892 2 24.012573700108067 25.647750785030997 25.992896570574132 24.3467789442868 3 24.488771068836538 25.222171025730873 24.98641039704844 25.302647508384148 4 24.39007896618675 25.228765776779383 25.83077849685639 24.550376760177485 5 23.581978513908542 25.612005395573195 25.9604899325327 24.845526157985567 6 23.425208101357704 25.428619286703114 26.101538032622805 25.044634579316376 7 23.677200981922972 25.77646831240407 25.788314618653157 24.758016087019797 8 23.761556356459423 25.55071468706755 26.02593392923524 24.661795027237783 9 23.760318769649356 25.3759915101399 26.12522996357743 24.738459756633315 10-11 23.794754515264284 25.480962168613313 26.21820909410637 24.50607422201603 12-13 23.92633205335287 25.283661829375465 26.100406252481662 24.689599864789997 14-15 23.95576783773995 25.28987270614512 26.048523708197514 24.70583574791742 16-17 24.03188639058612 25.22558880958214 25.98386675774459 24.758658042087152 18-19 24.140285657535024 25.115667930424944 25.88521240589484 24.858834006145187 20-21 24.217848104841615 25.135606950247926 25.825281929795878 24.82126301511459 22-23 24.27919477443652 25.16216469979282 25.757091240948714 24.80154928482195 24-25 24.361196142647877 25.117946822458727 25.61676479593932 24.904092238954075 26-27 24.379603275456645 25.168252491495064 25.599641929082072 24.85250230396622 28-29 24.44985084937475 25.152909337962885 25.521013231989258 24.876226580673105 30-31 24.488672936630707 25.12803307942317 25.444338864035387 24.938955119910737 32-33 24.496098375857464 25.1086886400565 25.45254237479963 24.942670609286406 34-35 24.516244493976355 25.085227976082265 25.448667389603063 24.949860140338316 36-37 24.561492699220288 25.071476981240526 25.380573512669248 24.98645680686994 38-39 24.569892610027257 25.056247152274235 25.38831638067035 24.985543857028155 40-41 24.567037322570233 25.039674058720813 25.38292799740744 25.010360621301515 42-43 24.645131052405304 24.977023077470147 25.304934699699356 25.072911170425193 44-45 24.63400321899377 24.98902722940124 25.322735274145717 25.05423427745927 46-47 24.60704781107322 25.06701797165321 25.28973842925512 25.03619578801845 48-49 24.63557250725445 24.959776342457644 25.2833246583129 25.121326491975005 50-51 24.63502030959428 24.99374336699049 25.310265098739738 25.060971224675495 52-53 24.688514278365414 25.036352085600257 25.227321677180274 25.047811958854055 54-55 24.687189008211433 24.954044780836824 25.24267911594316 25.116087095008577 56-57 24.66301314614692 25.00021693623037 25.22914125132968 25.107628666293035 58-59 24.677320960386083 25.044527292938657 25.195840862227968 25.08231088444729 60-61 24.707664854460486 24.981898610240542 25.181623447422353 25.128813087876615 62-63 24.695064686120737 24.99793566723267 25.209938280340776 25.097061366305816 64-65 24.708924791024348 25.0707495016563 25.15300939983097 25.067316307488376 66-67 24.773358289776276 25.12035753924379 25.032099823885417 25.07418434709451 68-69 24.74731886097766 25.039161962994662 25.113225078728313 25.100294097299365 70-71 24.803968807805603 25.055183770385337 25.11928914296073 25.021558278848328 72-73 24.823877739923255 25.026403955717186 25.08235261273249 25.067365691627074 74-75 24.799803208204285 25.034219739016955 25.109695580145306 25.05628147263345 76-77 24.88485561314825 25.08171708290165 25.05703157900396 24.976395724946133 78-79 24.860972406020064 25.061415382734136 25.02267886226249 25.05493334898331 80-81 24.858648462921643 25.08762367684319 25.062190900804953 24.991536959430217 82-83 24.86021114581997 25.106231217687906 25.041094645757735 24.992462990734392 84-85 24.900787142550598 25.070617850657495 25.002093816199807 25.0265011905921 86-87 24.91671771504886 25.060810902249912 25.022833366440256 24.999638016260974 88-89 24.910353953152196 25.055762677192302 25.029525040973503 25.004358328682002 90-91 24.954960866065537 25.069580037672118 24.973192065592055 25.00226703067029 92-93 24.935583514548245 25.174337802329937 24.947654479853032 24.942424203268786 94-95 24.95847064036249 25.286801007154658 24.90081973816411 24.853908614318744 96-97 25.01501613477447 25.242748376606798 24.83932082657736 24.902914662041372 98 25.091098544513358 25.223285962216856 24.854227128270626 24.831388364999157 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 21.0 2 20.0 3 24.5 4 36.0 5 50.5 6 55.5 7 78.5 8 103.5 9 113.0 10 141.5 11 172.5 12 212.5 13 274.0 14 357.0 15 521.5 16 795.5 17 1240.5 18 2009.5 19 3218.5 20 5325.0 21 8381.0 22 12464.0 23 18197.5 24 25255.5 25 34275.0 26 45390.0 27 58029.0 28 72291.5 29 87926.0 30 104429.0 31 121824.0 32 140582.0 33 160633.0 34 181529.5 35 203011.0 36 225072.0 37 248240.0 38 272336.5 39 293661.0 40 308936.0 41 319678.5 42 327045.0 43 329255.0 44 326920.5 45 320873.5 46 314124.5 47 308292.5 48 302711.5 49 299307.0 50 298241.0 51 297451.5 52 296549.0 53 297485.0 54 302093.5 55 308103.5 56 314794.5 57 319882.5 58 317868.5 59 309383.5 60 296986.0 61 281762.5 62 262165.5 63 238998.5 64 213661.5 65 187687.5 66 162198.0 67 137604.5 68 115703.5 69 96285.0 70 78836.5 71 63339.0 72 50197.0 73 39523.0 74 31066.5 75 24524.0 76 19243.5 77 15167.5 78 12085.0 79 9460.0 80 7308.0 81 5716.0 82 4394.0 83 3150.0 84 2153.5 85 1516.5 86 1086.5 87 737.5 88 468.5 89 305.0 90 187.5 91 107.5 92 71.0 93 53.5 94 42.5 95 26.0 96 22.5 97 20.0 98 18.0 99 61.5 100 104.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.008592097571520489 4 0.0 5 0.0 6 0.0 7 0.002876607858741766 8 0.010685890832637445 9 0.0 10-11 8.488351058582261E-5 12-13 0.0 14-15 0.004960969618682521 16-17 0.0011647903952610103 18-19 0.0 20-21 0.00495625386809442 22-23 0.0018532899811237935 24-25 4.715750588101256E-6 26-27 0.0037443059669523973 28-29 0.0068897125839209195 30-31 0.004937391797082346 32-33 0.0 34-35 0.0016127870814057108 36-37 0.005385389965188782 38-39 0.0 40-41 0.0010657603365312747 42-43 0.0077008261576456735 44-45 0.0035273849330959292 46-47 0.0 48-49 0.004437527581285272 50-51 0.0 52-53 0.00500342269952433 54-55 0.005347676297891412 56-57 0.005060017322896211 58-59 0.002192833847281714 60-61 0.005866425545887639 62-63 0.0 64-65 0.0029850920823096933 66-67 0.0019240424811602995 68-69 0.0020938131076567493 70-71 0.0052675589858142795 72-73 2.8294997227561857E-5 74-75 0.0027635354049913973 76-77 0.0011224845204432495 78-79 0.004707980437256278 80-81 0.0 82-83 0.0017142152027654492 84-85 0.0018054703103641274 86-87 0.002739645005411864 88-89 6.91831025105458E-4 90-91 0.004695262954427511 92-93 0.009804025882628329 94-95 0.0 96-97 0.0010450028084450476 98 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 26-27 1.0 28-29 1.0 30-31 0.0 32-33 0.0 34-35 2.0 36-37 3.0 38-39 0.0 40-41 0.0 42-43 2.0 44-45 3.0 46-47 3.0 48-49 2.0 50-51 5.0 52-53 7.0 54-55 5.0 56-57 11.0 58-59 10.0 60-61 8.0 62-63 11.0 64-65 12.0 66-67 11.0 68-69 26.0 70-71 42.0 72-73 135.0 74-75 623.0 76-77 1980.0 78-79 3886.0 80-81 6091.0 82-83 8423.0 84-85 11027.0 86-87 14331.0 88-89 18610.0 90-91 11207.0 92-93 0.0 94-95 0.0 96-97 324.0 98-99 1.0525964E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.7052883985996 #Duplication Level Percentage of total 1 57.36247417787218 2 24.24011865022966 3 10.507730811689582 4 4.458595959408516 5 1.921010472166561 6 0.7676734088962489 7 0.38633074447469073 8 0.16317719308027917 9 0.07785574413932593 >10 0.11373132206349969 >50 0.001301515979443532 >100 0.0 >500 0.0 >1k 0.0 >5k 0.0 >10k+ 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0.0 2.8294503528607536E-5 9.431501176202511E-6 0.0 0.07279232607793099 3.1123953881468286E-4 2 0.0 3.7726004704810046E-5 2.8294503528607536E-5 0.0 0.08289346383764387 3.8669154822430296E-4 3 9.431501176202511E-6 3.7726004704810046E-5 2.8294503528607536E-5 9.431501176202511E-6 0.09261734155030867 4.149860517529105E-4 4 9.431501176202511E-6 6.602050823341759E-5 4.715750588101256E-5 4.715750588101256E-5 0.10107739810536231 4.904380611625306E-4 5 1.8863002352405023E-5 7.545200940962009E-5 4.715750588101256E-5 4.715750588101256E-5 0.10899985909337243 5.375955670435432E-4 6 1.8863002352405023E-5 7.545200940962009E-5 6.602050823341759E-5 4.715750588101256E-5 0.11741275814254507 6.036160752769607E-4 7 1.8863002352405023E-5 7.545200940962009E-5 9.431501176202512E-5 4.715750588101256E-5 0.12574077368113187 6.413420799817708E-4 8 1.8863002352405023E-5 1.0374651293822762E-4 1.0374651293822762E-4 4.715750588101256E-5 0.13376698118208022 7.262255905675934E-4 9 1.8863002352405023E-5 1.3204101646683517E-4 1.1317801411443014E-4 4.715750588101256E-5 0.13987859394425944 8.394036046820235E-4 10-11 1.8863002352405023E-5 1.6505127058354395E-4 1.3675676705493642E-4 4.715750588101256E-5 0.15033341299807995 9.903076235012638E-4 12-13 3.7726004704810046E-5 1.8863002352405024E-4 1.603355199954427E-4 4.715750588101256E-5 0.16244346050832398 0.0012025163999658203 14-15 7.545200940962009E-5 1.9334577411215148E-4 1.7448277175974647E-4 6.130475764531633E-5 0.17265306053156318 0.0013345574164326554 16-17 1.178937647025314E-4 1.9806152470025276E-4 2.0749302587645525E-4 7.545200940962009E-5 0.18283908180186187 0.001433588178782782 18-19 1.4147251764303766E-4 2.310717788169615E-4 2.2164027764075901E-4 7.545200940962009E-5 0.19186974417807579 0.0015137559387805033 20-21 1.5090401881924018E-4 2.546505317574678E-4 2.263560282288603E-4 7.545200940962009E-5 0.2005938827660631 0.0016127867011306294 22-23 1.9806152470025273E-4 2.546505317574678E-4 2.4993478116936655E-4 8.959926117392387E-5 0.2084220287423112 0.0016976702117164522 24-25 2.687977835217716E-4 2.7351353410987283E-4 2.7351353410987283E-4 9.431501176202512E-5 0.21645766774443576 0.0017967009740665785 26-27 3.0180803763848037E-4 3.0180803763848037E-4 2.7351353410987283E-4 1.3204101646683517E-4 0.2249365873018418 0.0019240262399453124 28-29 3.395340423432904E-4 3.1123953881468286E-4 2.923765364622778E-4 1.3204101646683517E-4 0.2328543325392638 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