FastQCFastQC Report
Fri 13 Oct 2023
Trimmomatic on FASTQ splitter on data 54_ Reverse.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic on FASTQ splitter on data 54_ Reverse.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9677006
Total Bases239.5 Mbp
Sequences flagged as poor quality0
Sequence length4-25
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTCGGGTGCCAAGGAACTCCAG237475824.540214194348955RNA PCR Primer, Index 1 (100% over 25bp)
GGAATTCTCGGGTGCCAAGGAACTC7897208.160788574482645RNA PCR Primer, Index 1 (100% over 25bp)
AATTCTCGGGTGCCAAGGAACTCCA4030474.16499690090096RNA PCR Primer, Index 1 (100% over 25bp)
TTCTCGGGTGCCAAGGAACTCCAGT3817313.9447221588991472RNA PCR Primer, Index 1 (100% over 25bp)
TCTCGGGTGCCAAGGAACTCCAGTC3355193.4671777613861146RNA PCR Primer, Index 1 (100% over 25bp)
GAATTCTCGGGTGCCAAGGAACTCC3313743.4243442651580454RNA PCR Primer, Index 1 (100% over 25bp)
CTCGGGTGCCAAGGAACTCCAGTCA2941273.039442158039377RNA PCR Primer, Index 1 (100% over 25bp)
CGGGTGCCAAGGAACTCCAGTCACT2723342.8142382054945507RNA PCR Primer, Index 3 (100% over 25bp)
TCGGGTGCCAAGGAACTCCAGTCAC2474292.556875546010822RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACT2431002.512140635233666RNA PCR Primer, Index 1 (100% over 25bp)
GGTGCCAAGGAACTCCAGTCACTAT2156522.2284991866285915RNA PCR Primer, Index 44 (100% over 25bp)
GGGTGCCAAGGAACTCCAGTCACTA1938952.003667249973804RNA PCR Primer, Index 10 (100% over 25bp)
GATTGGAATTCTCGGGTGCCAAGGA1435661.483578701924955RNA PCR Primer, Index 1 (100% over 22bp)
GCCAAGGAACTCCAGTCACTATAAT1365931.4115212907794001RNA PCR Primer, Index 44 (100% over 25bp)
GTGCCAAGGAACTCCAGTCACTATA1343911.3887663188386987RNA PCR Primer, Index 44 (100% over 25bp)
TGCCAAGGAACTCCAGTCACTATAA1169511.208545287664387RNA PCR Primer, Index 44 (100% over 25bp)
CTGGAATTCTCGGGTGCCAAGGAAC985841.0187448473215786RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTCACTATAATATCTCGTATGC797360.8239738613368639RNA PCR Primer, Index 44 (100% over 25bp)
CAAGGAACTCCAGTCACTATAATAT776520.8024382748135115RNA PCR Primer, Index 44 (100% over 25bp)
CCAAGGAACTCCAGTCACTATAATA720070.7441041165004961RNA PCR Primer, Index 44 (100% over 25bp)
TGATTGGAATTCTCGGGTGCCAAGG707010.7306082067118693Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGAATTCTCGGGTGCCAAGGAAC534110.5519372417460524RNA PCR Primer, Index 1 (100% over 24bp)
GATGGAATTCTCGGGTGCCAAGGAA413980.4277976059950774RNA PCR Primer, Index 1 (100% over 23bp)
ATGGAATTCTCGGGTGCCAAGGAAC407460.42105998487548735RNA PCR Primer, Index 1 (100% over 24bp)
AAGGAACTCCAGTCACTATAATATC398360.4116562498772864RNA PCR Primer, Index 44 (100% over 25bp)
CTCGGCTGGAATTCTCGGGTGCCAA387890.40083678774199377No Hit
GTGGAATTCTCGGGTGCCAAGGAAC381100.39382015470487464RNA PCR Primer, Index 1 (100% over 24bp)
TCATTGCTACTGGAATTCTCGGGTG355770.3676447033307616No Hit
ACTGGAATTCTCGGGTGCCAAGGAA333470.34460038569780777RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGAATTCTCGGGTGCCAAGGAA317770.3283763593822304RNA PCR Primer, Index 1 (100% over 23bp)
AAGTTGCGCCCGAGGCCTTTGGAAT307970.3182492601533987No Hit
GCTGGAATTCTCGGGTGCCAAGGAA304970.31514912773640935RNA PCR Primer, Index 1 (100% over 23bp)
AGGAACTCCAGTCACTATAATATCT263770.27257397587642296RNA PCR Primer, Index 44 (100% over 25bp)
TGAACTGGAATTCTCGGGTGCCAAG226500.2340599974826925No Hit
TGTGGAATTCTCGGGTGCCAAGGAA218370.22565863863265148RNA PCR Primer, Index 1 (100% over 23bp)
ATTGGAATTCTCGGGTGCCAAGGAA215540.2227341803859582RNA PCR Primer, Index 1 (100% over 23bp)
AAGTTGCGCCCGAGTGGAATTCTCG202750.20951728251486051No Hit
TCTGGAATTCTCGGGTGCCAAGGAA174700.18053104441601048RNA PCR Primer, Index 1 (100% over 23bp)
AAGTTGCGCCCGATGGAATTCTCGG167110.17268770940102754No Hit
CTCTGGAATTCTCGGGTGCCAAGGA162610.16803751077554357RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGAATTCTCGGGTGCCAAGGAA156070.16127922210650691RNA PCR Primer, Index 1 (100% over 23bp)
GCTTGGAATTCTCGGGTGCCAAGGA146270.15115212287767518RNA PCR Primer, Index 1 (100% over 22bp)
AGTTGCGCCCGAGGCCTTTGGAATT143540.1483310023782149No Hit
AACTGGAATTCTCGGGTGCCAAGGA140710.14540654413152165RNA PCR Primer, Index 1 (100% over 22bp)
GCCCTGGAATTCTCGGGTGCCAAGG137330.14191372827504706Illumina Small RNA Adapter 2 (100% over 21bp)
TTGAACTGGAATTCTCGGGTGCCAA136170.14071501040714451No Hit
GGAACTCCAGTCACTATAATATCTC128000.13227231645821033RNA PCR Primer, Index 44 (100% over 25bp)
AGTTGCGCCCGATGGAATTCTCGGG126620.13084625554639523No Hit
CCTTGGAATTCTCGGGTGCCAAGGA125230.12940986085985687RNA PCR Primer, Index 1 (100% over 22bp)
TGATGGAATTCTCGGGTGCCAAGGA116290.12017146625722874RNA PCR Primer, Index 1 (100% over 22bp)
GCCTTGGAATTCTCGGGTGCCAAGG112110.1158519484228903Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGAACTGGAATTCTCGGGTGCCA104780.10827729155071311No Hit
TATGGAATTCTCGGGTGCCAAGGAA103920.1073885869245095RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)