##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename Nr2.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10602766 Total Bases 1 Gbp Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.166752336135684 32.0 32.0 32.0 32.0 32.0 2 31.110973966604565 32.0 32.0 32.0 32.0 32.0 3 34.63101534071392 37.0 32.0 37.0 32.0 37.0 4 35.24859522505731 37.0 37.0 37.0 32.0 37.0 5 34.746840305633455 37.0 37.0 37.0 27.0 37.0 6 38.837276423906744 41.0 37.0 41.0 37.0 41.0 7 37.852960821732744 41.0 37.0 41.0 32.0 41.0 8 38.80500503359218 41.0 41.0 41.0 37.0 41.0 9 39.368580047885615 41.0 41.0 41.0 37.0 41.0 10-11 39.59799060924291 41.0 41.0 41.0 37.0 41.0 12-13 39.66517968047206 41.0 41.0 41.0 37.0 41.0 14-15 39.72906654735189 41.0 41.0 41.0 37.0 41.0 16-17 39.676918032520945 41.0 41.0 41.0 37.0 41.0 18-19 39.688696704237366 41.0 41.0 41.0 37.0 41.0 20-21 39.71846497413976 41.0 41.0 41.0 37.0 41.0 22-23 39.7237847652207 41.0 41.0 41.0 37.0 41.0 24-25 39.70545648182748 41.0 41.0 41.0 37.0 41.0 26-27 39.69060455545279 41.0 41.0 41.0 37.0 41.0 28-29 39.694385219856784 41.0 41.0 41.0 37.0 41.0 30-31 39.634118493230915 41.0 41.0 41.0 37.0 41.0 32-33 39.68006023145281 41.0 41.0 41.0 37.0 41.0 34-35 39.655025113258176 41.0 41.0 41.0 37.0 41.0 36-37 39.62619329710756 41.0 41.0 41.0 37.0 41.0 38-39 39.60815602268314 41.0 41.0 41.0 37.0 41.0 40-41 39.64637185240153 41.0 41.0 41.0 37.0 41.0 42-43 39.559797556599854 41.0 41.0 41.0 37.0 41.0 44-45 39.35910968892457 41.0 41.0 41.0 37.0 41.0 46-47 39.27724232525739 41.0 41.0 41.0 37.0 41.0 48-49 39.24590847331724 41.0 41.0 41.0 37.0 41.0 50-51 39.23377121592611 41.0 41.0 41.0 37.0 41.0 52-53 39.24109307891922 41.0 41.0 41.0 37.0 41.0 54-55 39.22640931621051 41.0 41.0 41.0 37.0 41.0 56-57 39.19644510687117 41.0 41.0 41.0 37.0 41.0 58-59 39.16700458163464 41.0 41.0 41.0 37.0 41.0 60-61 39.14492058015804 41.0 41.0 41.0 37.0 41.0 62-63 39.12318356360973 41.0 41.0 41.0 37.0 41.0 64-65 39.15374686190377 41.0 41.0 41.0 37.0 41.0 66-67 39.11766891771449 41.0 41.0 41.0 37.0 41.0 68-69 39.0469360542334 41.0 41.0 41.0 37.0 41.0 70-71 38.9369096233945 41.0 41.0 41.0 34.5 41.0 72-73 39.07148615748004 41.0 41.0 41.0 37.0 41.0 74-75 39.066162075066075 41.0 41.0 41.0 37.0 41.0 76-77 38.33805480569882 41.0 39.0 41.0 34.5 41.0 78-79 38.68568244361896 41.0 39.0 41.0 32.0 41.0 80-81 39.029133765660774 41.0 41.0 41.0 37.0 41.0 82-83 39.16111385463001 41.0 41.0 41.0 37.0 41.0 84-85 39.08286243419877 41.0 41.0 41.0 37.0 41.0 86-87 39.046002477089466 41.0 41.0 41.0 37.0 41.0 88-89 39.03622700906537 41.0 41.0 41.0 37.0 41.0 90-91 39.06791109980169 41.0 41.0 41.0 37.0 41.0 92-93 39.024423673973374 41.0 41.0 41.0 37.0 41.0 94-95 38.96524562552828 41.0 41.0 41.0 37.0 41.0 96-97 38.90602692731312 41.0 41.0 41.0 37.0 41.0 98-99 38.85380380930788 41.0 41.0 41.0 37.0 41.0 100-101 37.994161523511885 41.0 39.0 41.0 29.5 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.08807782327965796 1101 2 -0.23449243752127913 1101 3 -0.6493233112001988 1101 4 -0.3207938008879765 1101 5 -0.35568316500863517 1101 6 -0.4592689197514588 1101 7 -0.42304104903283957 1101 8 -0.33208147249136744 1101 9 -0.5080763151090437 1101 10-11 -0.5427196744596259 1101 12-13 -0.6312212994254196 1101 14-15 -0.559482049045485 1101 16-17 -0.5179041072671211 1101 18-19 -0.5037555334273662 1101 20-21 -0.6844592282859736 1101 22-23 -0.6498404438556733 1101 24-25 -0.6555712079516525 1101 26-27 -0.7111955506714978 1101 28-29 -0.5489868238180478 1101 30-31 -0.7142676545859459 1101 32-33 -0.8832548525769894 1101 34-35 -0.7699664894712086 1101 36-37 -0.7766209398046655 1101 38-39 -0.7182150253441861 1101 40-41 -0.7383723302821039 1101 42-43 -0.8529892987055447 1101 44-45 -0.8718514056967805 1101 46-47 -0.8527327301408434 1101 48-49 -0.889169363920665 1101 50-51 -0.9987864744984734 1101 52-53 -0.9051350130733837 1101 54-55 -0.9411729514216987 1101 56-57 -0.9765492345091715 1101 58-59 -0.998192220713932 1101 60-61 -1.0052583297586963 1101 62-63 -1.1794026048324113 1101 64-65 -1.110646983721118 1101 66-67 -1.1813133743669866 1101 68-69 -1.0417633789442604 1101 70-71 -0.8797083673398944 1101 72-73 -1.0696212520233317 1101 74-75 -1.1193745364266192 1101 76-77 -1.4811024231780223 1101 78-79 -1.196901362198993 1101 80-81 -0.9857589662124226 1101 82-83 -1.0898152203003804 1101 84-85 -1.1898226984800928 1101 86-87 -1.1134991532182994 1101 88-89 -1.0468536539957753 1101 90-91 -1.100192311517553 1101 92-93 -1.2293037437016068 1101 94-95 -1.2878827866669695 1101 96-97 -1.228021040625876 1101 98-99 -1.4625713235344477 1101 100-101 -1.6779781968608987 1102 1 -0.007204969752198309 1102 2 -0.08497125725447319 1102 3 0.021458107516835412 1102 4 -0.20048654576224578 1102 5 0.10799864379531243 1102 6 -0.203779689201518 1102 7 -0.09243010841507271 1102 8 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1103 90-91 -0.31253690515330135 1103 92-93 -0.25582392575953605 1103 94-95 -0.3543455224908314 1103 96-97 -0.43862828879530014 1103 98-99 -0.4350985512362797 1103 100-101 -0.24306237620115922 1104 1 -0.07909623741771554 1104 2 -0.0022822911016042724 1104 3 0.01889241865718816 1104 4 -0.05910193705341982 1104 5 0.011271917702778467 1104 6 -0.09918376451949484 1104 7 0.01219170798998448 1104 8 -0.12450365644430406 1104 9 -0.23162449717047906 1104 10-11 -0.3248756670095929 1104 12-13 -0.19493215075489445 1104 14-15 -0.2235990771772265 1104 16-17 -0.16895268189504264 1104 18-19 -0.16016865458698248 1104 20-21 -0.17350423021300543 1104 22-23 -0.19858232945111354 1104 24-25 -0.229380348996159 1104 26-27 -0.22555709718420758 1104 28-29 -0.30693299203340274 1104 30-31 -0.2436376540879408 1104 32-33 -0.3184894235218749 1104 34-35 -0.27850698854624767 1104 36-37 -0.3191603228086066 1104 38-39 -0.3201313724109127 1104 40-41 -0.35288587108934877 1104 42-43 -0.29944951895605953 1104 44-45 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2223 86-87 -0.2445138029982985 2223 88-89 0.28853032756641284 2223 90-91 0.4045807679286 2223 92-93 0.38647102528132393 2223 94-95 0.3724293970210226 2223 96-97 0.3162729049658566 2223 98-99 0.4010732086541111 2223 100-101 0.4255416937989267 2224 1 -0.016349089999177835 2224 2 0.07244721723868963 2224 3 0.09943517794594214 2224 4 0.15154487718795195 2224 5 0.16556629360032815 2224 6 0.203299399801125 2224 7 -0.02573732108247384 2224 8 0.09389201577783268 2224 9 0.20377292197579777 2224 10-11 0.2795387697365257 2224 12-13 0.24471699663570234 2224 14-15 0.23080442882127272 2224 16-17 0.2647467243192523 2224 18-19 0.2286636466812766 2224 20-21 0.28452312902701493 2224 22-23 0.24701372111235997 2224 24-25 0.22743191242031457 2224 26-27 0.2776877052231299 2224 28-29 0.21345883868500692 2224 30-31 0.2799983360526568 2224 32-33 0.23176197258735698 2224 34-35 0.3081089539824049 2224 36-37 0.16179910194583158 2224 38-39 0.33960306806894636 2224 40-41 0.27838585598872356 2224 42-43 0.29771547081271166 2224 44-45 0.33541410358437673 2224 46-47 -0.902780198367445 2224 48-49 -0.4379821196579101 2224 50-51 0.2796996698158267 2224 52-53 0.33871667268323336 2224 54-55 0.3997922369984295 2224 56-57 0.3821595821703099 2224 58-59 0.47496487960626155 2224 60-61 0.4515890729423191 2224 62-63 0.45787108024843093 2224 64-65 0.45338934997045754 2224 66-67 0.4857335117729278 2224 68-69 0.5121962028448834 2224 70-71 0.6681860591299653 2224 72-73 0.4948938320663956 2224 74-75 0.4286862308104986 2224 76-77 0.5589095417143355 2224 78-79 0.5549775963156307 2224 80-81 0.5024854267698373 2224 82-83 0.33323379662402175 2224 84-85 0.40374818588657035 2224 86-87 0.4385943219159856 2224 88-89 0.40053188065448353 2224 90-91 0.31450654328032357 2224 92-93 0.37754745496781084 2224 94-95 0.3358387813149335 2224 96-97 0.30502849942594423 2224 98-99 0.3952952121650952 2224 100-101 0.4595341729825009 2225 1 -3.4424046869929725E-5 2225 2 0.09472095397166314 2225 3 0.18609026801247808 2225 4 0.14876778498604182 2225 5 -0.18915043438288848 2225 6 0.1656116511665573 2225 7 -0.02307320725104489 2225 8 0.07715149955846812 2225 9 0.19787361527997405 2225 10-11 0.1504875432809527 2225 12-13 0.16360795471721445 2225 14-15 0.1729981900128763 2225 16-17 0.10813148377504689 2225 18-19 0.17326990969446854 2225 20-21 0.1900156795936212 2225 22-23 0.11456124511827426 2225 24-25 0.2088245787755838 2225 26-27 0.09456772763296328 2225 28-29 0.14635257623840658 2225 30-31 0.14060681239938333 2225 32-33 0.23242522474624394 2225 34-35 0.24093627566525555 2225 36-37 0.3113992695663015 2225 38-39 0.29588303368363 2225 40-41 0.31600525100938626 2225 42-43 0.2084140805555066 2225 44-45 0.2595374673527502 2225 46-47 0.2846309402346918 2225 48-49 0.35413170678690875 2225 50-51 0.30124759134243106 2225 52-53 0.34472502560364404 2225 54-55 0.3004231254435652 2225 56-57 0.32219376804273026 2225 58-59 0.36499796629092884 2225 60-61 0.3321355233704324 2225 62-63 0.32865939504402064 2225 64-65 0.3716737377194832 2225 66-67 0.3900423277800016 2225 68-69 0.42023374701918925 2225 70-71 0.5273412506164021 2225 72-73 0.3547142361187454 2225 74-75 0.30788551899010486 2225 76-77 0.4287769184789596 2225 78-79 -0.05609706029019179 2225 80-81 -2.5838086197799726 2225 82-83 -0.3149357498012506 2225 84-85 0.21278562830102743 2225 86-87 0.33190956057910626 2225 88-89 0.24421985728552187 2225 90-91 0.31736611989133223 2225 92-93 0.30456153522301577 2225 94-95 0.2742337836141644 2225 96-97 0.25013870036426766 2225 98-99 0.2449012496748253 2225 100-101 0.4461657948238411 2226 1 0.09199800734742425 2226 2 0.013157696848853817 2226 3 -0.17963567790604884 2226 4 0.008645517577974715 2226 5 -0.25016659207906855 2226 6 0.11353979147096283 2226 7 -0.1945980210593632 2226 8 -0.10193708631141618 2226 9 0.07159219720372789 2226 10-11 0.10805410556647388 2226 12-13 0.13128022427714825 2226 14-15 0.13831664997672988 2226 16-17 0.13039064128665245 2226 18-19 0.21370770235481018 2226 20-21 0.2571206688229424 2226 22-23 0.12162179874400891 2226 24-25 0.12979632420584153 2226 26-27 0.17193482082294054 2226 28-29 0.1408432223986793 2226 30-31 0.24877341354898164 2226 32-33 0.1442464770275862 2226 34-35 0.23004551331086276 2226 36-37 0.23639236951061093 2226 38-39 0.22881131778925123 2226 40-41 0.29564435527404953 2226 42-43 0.3089344556082807 2226 44-45 0.261804018099852 2226 46-47 0.3176350854847598 2226 48-49 0.39665528623859103 2226 50-51 0.34166760619205405 2226 52-53 0.3809778426830732 2226 54-55 0.35435171959694145 2226 56-57 0.3069899818220705 2226 58-59 0.3814830762075587 2226 60-61 0.42375726331290764 2226 62-63 0.34518534587719074 2226 64-65 0.3791172693179661 2226 66-67 0.4188989406759944 2226 68-69 0.49291437935738713 2226 70-71 0.5586669199973073 2226 72-73 0.35678238237975535 2226 74-75 0.38259290755120645 2226 76-77 0.44770683773132447 2226 78-79 0.38255083973509585 2226 80-81 -2.4485999436317556 2226 82-83 -0.19375843384843705 2226 84-85 0.327633528834248 2226 86-87 0.365709228539032 2226 88-89 0.3831766484881527 2226 90-91 0.3460221489750026 2226 92-93 0.3708702810204443 2226 94-95 0.2927284039044906 2226 96-97 0.2789857207928961 2226 98-99 0.3712144481617443 2226 100-101 0.5066109690508469 2227 1 0.04211065620484433 2227 2 0.09415758956422948 2227 3 -0.03265574013113337 2227 4 0.012936902622499247 2227 5 -0.06563703516457764 2227 6 0.2040880159104006 2227 7 -0.021869773017307637 2227 8 0.054054760056999385 2227 9 0.17265431500224793 2227 10-11 0.20037252633677838 2227 12-13 0.20203443519872621 2227 14-15 0.2594946351714498 2227 16-17 0.29378512685687497 2227 18-19 0.2763619240048456 2227 20-21 0.3174146286984936 2227 22-23 0.23148125588379997 2227 24-25 0.20596840874613065 2227 26-27 0.1919970459060778 2227 28-29 0.19384182769854164 2227 30-31 0.3168991511307908 2227 32-33 0.22519272742989926 2227 34-35 0.2516633654726519 2227 36-37 0.2224453681159133 2227 38-39 0.26845298713803345 2227 40-41 0.2524086509504784 2227 42-43 0.24279348360956732 2227 44-45 0.3008019796919541 2227 46-47 0.40206808623575796 2227 48-49 0.44917111072093974 2227 50-51 0.37363842477375186 2227 52-53 0.35329840914589994 2227 54-55 0.29786636394840826 2227 56-57 0.3497965476411835 2227 58-59 0.3962883546111655 2227 60-61 0.42627845202656545 2227 62-63 0.47961298239687267 2227 64-65 0.4496032686742524 2227 66-67 0.45741412145059 2227 68-69 0.46201640703682045 2227 70-71 0.6880521790896807 2227 72-73 0.5011874891279504 2227 74-75 0.3965399089459041 2227 76-77 0.6440913458313062 2227 78-79 0.611067093355949 2227 80-81 0.527100088553631 2227 82-83 0.3822527237526785 2227 84-85 0.3925943699457193 2227 86-87 0.45952963407491154 2227 88-89 0.43215208030877506 2227 90-91 0.3859320298890694 2227 92-93 0.3610537377310905 2227 94-95 0.3317800078096482 2227 96-97 0.34914986627084943 2227 98-99 0.43376138518567586 2227 100-101 0.5081665961294846 2228 1 -0.003474759967801333 2228 2 0.038489690828580336 2228 3 0.022378351547068576 2228 4 0.059818336555380824 2228 5 -0.18514740176474476 2228 6 -6.240208296760827 2228 7 -3.0821859688894975 2228 8 -1.02485624742679 2228 9 -0.2612766147983834 2228 10-11 0.1496600087204243 2228 12-13 0.15104567184312145 2228 14-15 0.21582005395073622 2228 16-17 0.26761343996761866 2228 18-19 0.18457189219114412 2228 20-21 0.2604150859160299 2228 22-23 0.20834007564579338 2228 24-25 0.22777502341952527 2228 26-27 0.1761628251617111 2228 28-29 0.16079240097337077 2228 30-31 0.25936708057482605 2228 32-33 0.2540501818692391 2228 34-35 0.19963267525270822 2228 36-37 0.27599435490919433 2228 38-39 0.29148688274992907 2228 40-41 -0.6140038112057269 2228 42-43 0.12809079917293076 2228 44-45 -0.022070128756084273 2228 46-47 0.061311338462886056 2228 48-49 0.351191518821274 2228 50-51 0.36130288390910437 2228 52-53 0.38528602645498466 2228 54-55 0.3617494896023956 2228 56-57 0.4756563462739578 2228 58-59 0.4132018327251856 2228 60-61 0.4113062413416344 2228 62-63 0.43561841382308586 2228 64-65 0.44579304138179054 2228 66-67 0.46206525895166095 2228 68-69 0.4607595863071552 2228 70-71 -2.87075521514204 2228 72-73 -1.450972981405215 2228 74-75 0.18841422654832485 2228 76-77 0.53614385978927 2228 78-79 0.5796154122578017 2228 80-81 0.4392381997371828 2228 82-83 0.32359010695891044 2228 84-85 0.3874726246179918 2228 86-87 -5.248418647269197 2228 88-89 -1.0525532059017735 2228 90-91 -0.24502200311207645 2228 92-93 0.2044853785155425 2228 94-95 0.2914884630554866 2228 96-97 0.34851378692177093 2228 98-99 0.3362287043571115 2228 100-101 0.5281703908565021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 7.0 14 71.0 15 433.0 16 1900.0 17 4886.0 18 8383.0 19 11230.0 20 14072.0 21 17300.0 22 21310.0 23 25462.0 24 29570.0 25 34557.0 26 39942.0 27 46642.0 28 53805.0 29 63338.0 30 74811.0 31 88516.0 32 105804.0 33 129342.0 34 159872.0 35 205445.0 36 276069.0 37 398384.0 38 690176.0 39 1858310.0 40 6243128.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.435640438081865 27.30965734299278 22.716989099320035 26.53771311960532 2 22.28222612269587 26.60601625105114 26.833233620321906 24.278524005931086 3 23.44085496180902 24.92569391798329 25.568412997136786 26.065038123070906 4 23.473884078928084 24.80059448638214 25.771765593996886 25.953755840692892 5 24.012573700108067 25.647750785030997 25.992896570574132 24.3467789442868 6 24.488771068836538 25.222171025730873 24.98641039704844 25.302647508384148 7 24.39007896618675 25.228765776779383 25.83077849685639 24.550376760177485 8 23.581978513908542 25.612005395573195 25.9604899325327 24.845526157985567 9 23.425208101357704 25.428619286703114 26.101538032622805 25.044634579316376 10-11 23.719377022192035 25.663595907469567 25.9071196345385 24.709907435799895 12-13 23.78808643827261 25.419303677335996 26.163855306690515 24.628754577700875 14-15 23.825702651553378 25.401164186779184 26.181776528879354 24.591356632788084 16-17 23.952617647130946 25.326362950950728 26.07726606434585 24.64375333757248 18-19 24.020117446879148 25.177985972775563 26.005358301641916 24.796538278703366 20-21 24.06026139238402 25.184716923163258 25.95518994439358 24.79983174005914 22-23 24.168613228496373 25.182540426242927 25.865061257570982 24.78378508768972 24-25 24.273903604215942 25.11175239171976 25.73467686300005 24.87966714106425 26-27 24.299281904363447 25.13387072769502 25.713827882271474 24.85301948567006 28-29 24.361617878118462 25.167899983857378 25.6223974859957 24.84808465202846 30-31 24.474652991311103 25.120731481865754 25.515631897257506 24.88898362956563 32-33 24.437859251980573 25.128303099779497 25.52094189924806 24.912895748991865 34-35 24.47114271879621 25.168107076964635 25.458993436241073 24.901756767998087 36-37 24.516804612455726 25.072079052345202 25.414339602710466 24.996776732488605 38-39 24.520997634013614 25.105217827121717 25.436136193140545 24.937648345724124 40-41 24.58426769173742 25.071454543139417 25.39528371247652 24.948994052646643 42-43 24.607446773794685 24.99443541430604 25.338119317166857 25.05999849473241 44-45 24.559993152653618 25.020972356196886 25.37908574249969 25.039948748649802 46-47 24.63837704961175 25.03416235748234 25.319303790632734 25.008156802273167 48-49 24.650176189873473 25.000759235844683 25.264897857785414 25.08416671649643 50-51 24.604329662655953 24.98477755710161 25.32358537385433 25.087307406388103 52-53 24.61947980982043 25.015606290166126 25.30309120322104 25.061822696792408 54-55 24.708699405894162 24.984777974418698 25.234343266568644 25.0721793531185 56-57 24.66839534671174 24.987957811831365 25.248047382886124 25.09559945857077 58-59 24.666341480116312 25.017634445640795 25.252573318004952 25.063450756237938 60-61 24.686690098425743 25.004641567040192 25.174421294325906 25.134247040208162 62-63 24.660582589801315 25.003374296172755 25.223441601001905 25.11260151302403 64-65 24.717257566513194 25.02239765809955 25.201418585939333 25.058926189447924 66-67 24.724337975393674 25.00858761670387 25.13841106549074 25.128663342411716 68-69 24.69065536257813 25.097591043199053 25.133907827990765 25.07784576623205 70-71 24.77830813699987 25.104895777637832 25.072121005026375 25.04467508033592 72-73 24.80880474802368 24.99387652682449 25.085386728573244 25.11193199657859 74-75 24.7976930461453 25.028305430020886 25.128897702987473 25.045103820846343 76-77 24.802866271338953 25.078218987133027 25.09810059442516 25.02081414710286 78-79 24.85607441517233 25.044758928064464 25.062769079395007 25.0363975773682 80-81 24.85671425566772 25.08578564294196 25.042833873610128 25.01466622778019 82-83 24.863157711065163 25.082828791573263 25.057108303110343 24.99690519425123 84-85 24.886243538073195 25.092655474709964 25.01492914298396 25.00617184423288 86-87 24.873896199743932 25.13062628653888 25.01497041992158 24.980507093795612 88-89 24.94102674974097 25.15958417828137 24.967110278433534 24.93227879354413 90-91 24.970920123783646 25.098199108471782 24.956720901001283 24.97415986674329 92-93 24.978210675066816 25.15868881693845 24.944464455129175 24.918636052865555 94-95 25.029603164097097 25.217264752996027 24.87045889301969 24.882673189887182 96-97 25.071688575452434 25.23059616805098 24.822772334289056 24.87494292220753 98-99 25.10992886195923 25.278026507422684 24.796699276396367 24.81534535422172 100-101 25.20356535048553 25.323103285529474 24.72113159811863 24.752199765866365 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14.0 1 16.5 2 16.0 3 18.0 4 28.5 5 38.5 6 44.5 7 63.0 8 86.0 9 104.0 10 119.5 11 148.5 12 184.5 13 235.5 14 302.5 15 412.0 16 619.0 17 977.5 18 1586.0 19 2611.5 20 4234.5 21 6667.0 22 10197.0 23 15115.5 24 21557.5 25 29225.5 26 38947.0 27 50534.5 28 63723.5 29 77991.5 30 93557.5 31 110410.0 32 127620.0 33 145947.5 34 165710.5 35 185868.5 36 205840.0 37 227166.5 38 250244.0 39 272481.0 40 291302.5 41 305294.5 42 314572.5 43 319869.5 44 321037.5 45 318016.0 46 312732.5 47 306492.0 48 299745.5 49 295462.5 50 293716.0 51 291732.5 52 290054.5 53 291227.5 54 295080.5 55 300328.0 56 307202.0 57 312374.0 58 310693.0 59 303191.0 60 292220.5 61 278448.5 62 261027.0 63 239671.0 64 216405.5 65 191157.5 66 165882.0 67 142370.0 68 120360.0 69 100647.0 70 83443.5 71 67986.5 72 54236.0 73 42938.0 74 33840.0 75 26763.0 76 21250.0 77 16867.0 78 13347.0 79 10603.0 80 8404.5 81 6550.5 82 5091.5 83 3813.5 84 2650.0 85 1877.5 86 1327.5 87 934.5 88 649.5 89 408.5 90 253.5 91 149.0 92 88.0 93 64.5 94 50.5 95 31.5 96 22.0 97 18.5 98 21.0 99 31.0 100 52.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11538498538966153 2 0.022107438757018687 3 0.0 4 0.0 5 0.0 6 0.008592097571520489 7 0.0 8 0.0 9 0.0 10-11 0.006781249345689605 12-13 8.488351058582261E-5 14-15 0.0 16-17 0.0 18-19 0.004960969618682521 20-21 0.0011647903952610103 22-23 0.00495625386809442 24-25 0.0018532899811237935 26-27 0.0 28-29 0.003749021717540498 30-31 0.006847269853923024 32-33 0.004979832621034926 34-35 0.0 36-37 0.0016127867011306296 38-39 0.0 40-41 0.005385387171611635 42-43 0.0 44-45 0.0011176328893799975 46-47 0.011176328893799976 48-49 0.0 50-51 0.0 52-53 0.0044375213034032815 54-55 0.005003411373975432 56-57 0.004310196037524548 58-59 0.006097465510414924 60-61 0.0019287419905334136 62-63 0.005234483152792394 64-65 8.959926117392387E-4 66-67 0.0027351353410987283 68-69 0.002173961021114679 70-71 9.337186164440487E-4 72-73 0.001160074644672909 74-75 0.005281640658673406 76-77 1.4147251764303766E-5 78-79 0.0038857784845954353 80-81 0.001659944207011642 82-83 0.0030510906305015123 84-85 0.001711817463480756 86-87 0.00180141672465468 88-89 0.002730419590510627 90-91 6.884995858627834E-4 92-93 9.148556140916436E-4 94-95 0.004116850263412396 96-97 0.009497521684435928 98-99 0.0 100-101 0.0025842313222794885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0602766E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.6838422116555 #Duplication Level Percentage of total 1 57.31811252632203 2 24.28598063944757 3 10.516716251238755 4 4.445418154779839 5 1.9240524837214124 6 0.7717595771155199 7 0.3791375621956633 8 0.1611114827740786 9 0.07681767234357945 >10 0.11949174499439828 >50 0.0014019050671515338 >100 0.0 >500 0.0 >1k 0.0 >5k 0.0 >10k+ 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0.0 9.431501176202511E-6 0.0 0.0 0.0751030438661006 2.0749302587645525E-4 2 0.0 1.8863002352405023E-5 0.0 9.431501176202511E-6 0.08782613895279778 2.829450352860753E-4 3 0.0 2.8294503528607536E-5 9.431501176202511E-6 9.431501176202511E-6 0.09804988622780131 4.527120564577206E-4 4 0.0 5.658900705721507E-5 1.8863002352405023E-5 9.431501176202511E-6 0.10875464006279116 5.753215717483533E-4 5 0.0 6.602050823341759E-5 3.7726004704810046E-5 9.431501176202511E-6 0.11885577782250405 6.507735811579733E-4 6 9.431501176202511E-6 6.602050823341759E-5 3.7726004704810046E-5 1.8863002352405023E-5 0.12857965553516884 6.790680846865809E-4 7 9.431501176202511E-6 9.431501176202512E-5 5.658900705721507E-5 5.658900705721507E-5 0.1370397120902225 7.545200940962009E-4 8 1.8863002352405023E-5 1.0374651293822762E-4 5.658900705721507E-5 5.658900705721507E-5 0.14496217307823261 8.016775999772135E-4 9 1.8863002352405023E-5 1.0374651293822762E-4 7.545200940962009E-5 5.658900705721507E-5 0.15337507212740525 8.676981082106311E-4 10-11 1.8863002352405023E-5 1.178937647025314E-4 1.0846226352632888E-4 5.658900705721507E-5 0.16571619141646624 9.478658682083524E-4 12-13 1.8863002352405023E-5 1.697670211716452E-4 1.3204101646683517E-4 5.658900705721507E-5 0.17932113186313836 0.0011553588940848077 14-15 1.8863002352405023E-5 2.1220877646455652E-4 1.603355199954427E-4 5.658900705721507E-5 0.19273744228628642 0.001362851919961263 16-17 6.602050823341759E-5 2.1692452705265777E-4 1.6976702117164522E-4 6.130475764531633E-5 0.20337617561304286 0.0015467661928972119 18-19 8.959926117392386E-5 2.263560282288603E-4 2.0749302587645528E-4 8.016775999772135E-5 0.2136565118951036 0.0016363654540711356 20-21 1.3675676705493642E-4 2.3107177881696153E-4 2.2164027764075901E-4 8.488351058582261E-5 0.22317761233248 0.0017448277175974645 22-23 1.461882682311389E-4 2.829450352860753E-4 2.3578752940506278E-4 8.488351058582261E-5 0.23221299045928206 0.0018249954775951859 24-25 1.5090401881924018E-4 2.829450352860753E-4 2.452190305812653E-4 8.959926117392387E-5 0.24036652322610913 0.0019240262399453124 26-27 2.5465053175746785E-4 2.923765364622778E-4 2.7351353410987283E-4 1.0374651293822762E-4 0.24802018643059745 0.002013625501119236 28-29 2.782292846979741E-4 3.159552894027841E-4 2.829450352860753E-4 1.2260951529063265E-4 0.25666415725858704 0.0021173720140574637 30-31 3.301025411670879E-4 3.348182917551892E-4 2.8766078587417663E-4 1.4147251764303766E-4 0.2646620702560068 0.0022588445317005016 32-33 3.8669154822430296E-4 3.3953404234329045E-4 3.159552894027841E-4 1.4147251764303766E-4 0.2721601136910878 0.0024191800516959443 34-35 4.527120564577206E-4 3.536812941075942E-4 3.4896554351949294E-4 1.4147251764303766E-4 0.2793374860861779 0.0025653683199270833 36-37 5.045853129268344E-4 4.149860517529105E-4 3.67828545871898E-4 1.6976702117164522E-4 0.286467700975387 0.0027728613458035383 38-39 5.941845741007582E-4 4.149860517529105E-4 3.725442964599992E-4 1.6976702117164522E-4 0.2923812522128659 0.0029614913693275886 40-41 6.602050823341758E-4 4.2441755292911304E-4 3.9140729881240427E-4 1.7919852234784772E-4 0.29799771116329454 0.0031029638869706265 42-43 7.545200940962009E-4 4.3384905410531554E-4 4.2441755292911304E-4 1.7919852234784772E-4 0.30343497159137534 0.003291593910494677 44-45 7.922460988010109E-4 4.6214355763392307E-4 4.4799630586961933E-4 1.83914272935949E-4 0.30857042398181755 0.003423634926961512 46-47 8.441193552701248E-4 4.8572231057442936E-4 4.810065599863281E-4 2.2635602822886026E-4 0.3140925679204841 0.003560391694016448 48-49 9.195713646797448E-4 5.093010635149356E-4 5.564585693959482E-4 2.3578752940506278E-4 0.319534544099153 0.0036688539575427774 50-51 0.0010186021270298714 5.140168141030369E-4 6.036160752769607E-4 2.452190305812653E-4 0.3251934448048745 0.0038244737269501183 52-53 0.0010987698870275927 5.187325646911382E-4 6.177633270412644E-4 2.452190305812653E-4 0.3311965953035274 0.004060261256355181 54-55 0.0011789376470253139 5.281640658673407E-4 6.319105788055682E-4 2.7351353410987283E-4 0.3371148622915945 0.004281901533995941 56-57 0.0012355266540825288 5.470270682197457E-4 6.602050823341758E-4 2.829450352860753E-4 0.3426275747290849 0.004508257562224801 58-59 0.0012968314117278454 5.800373223364544E-4 7.262255905675934E-4 3.206710399908854E-4 0.34828175968421826 0.004753476592806066 60-61 0.0013487046681969592 6.036160752769607E-4 7.450885929199985E-4 3.67828545871898E-4 0.35461029697345015 0.004946822366918217 62-63 0.0014052936752541744 6.08331825865062E-4 7.828145976248085E-4 4.0083879998860676E-4 0.3605710057168101 0.005192041397499483 64-65 0.0015514819434853133 6.177633270412644E-4 8.29972103505821E-4 4.1498605175291055E-4 0.3664751254531129 0.00544197617866885 66-67 0.001641081204659237 6.27194828217467E-4 8.488351058582261E-4 4.574278070458218E-4 0.37326580629997874 0.005640037703369101 68-69 0.0017071017128926546 6.413420799817708E-4 8.771296093868336E-4 4.6214355763392307E-4 0.3807638497350597 0.005922982738655177 70-71 0.0017731222211260721 6.554893317460746E-4 9.007083623273399E-4 5.045853129268344E-4 0.38850239645013385 0.006154054517472139 72-73 0.0018957317364167049 6.696365835103783E-4 9.101398635035425E-4 5.187325646911382E-4 0.3949582590052445 0.006488872809227328 74-75 0.0022069712752313874 6.837838352746822E-4 9.242871152678461E-4 5.470270682197457E-4 0.4019422856262225 0.006818975350394416 76-77 0.0031029638869706265 7.167940893913909E-4 9.337186164440486E-4 5.753215717483533E-4 0.4095016338189488 0.007139646390385302 78-79 0.007271687406852137 7.35657091743796E-4 9.337186164440486E-4 5.800373223364544E-4 0.416966666999913 0.007554632442138212 80-81 0.02151796993350603 7.498043435080998E-4 9.384343670321498E-4 5.941845741007582E-4 0.42501173750321375 0.007941323990362514 82-83 0.05300975236084622 7.592358446843021E-4 9.57297369384555E-4 6.036160752769607E-4 0.433033229253574 0.008398751797408337 84-85 0.10350600965823446 7.828145976248084E-4 9.620131199726562E-4 6.130475764531632E-4 0.440809501973353 0.008794874846808843 86-87 0.17490718931267557 7.828145976248084E-4 9.855918729131624E-4 6.602050823341758E-4 0.4489960449942968 0.00927116565620707 88-89 0.2687600575170668 8.11109101153416E-4 9.95023374089365E-4 6.790680846865808E-4 0.4557537155870459 0.009695583209136181 90 0.35757650409336583 8.11109101153416E-4 0.0010186021270298712 6.979310870389859E-4 0.46090803097984057 0.010053980253831878 >>END_MODULE