##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Tr2.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16293448
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	101
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28795765021621	32.0	32.0	32.0	32.0	32.0
2	31.44766356390618	32.0	32.0	32.0	32.0	32.0
3	34.95870923085157	37.0	32.0	37.0	32.0	37.0
4	35.620306150054915	37.0	37.0	37.0	32.0	37.0
5	35.93960535547786	37.0	37.0	37.0	32.0	37.0
6	39.48841196780448	41.0	41.0	41.0	37.0	41.0
7	39.64261033023826	41.0	41.0	41.0	37.0	41.0
8	39.79960773189321	41.0	41.0	41.0	37.0	41.0
9	39.95868701333198	41.0	41.0	41.0	37.0	41.0
10-11	39.99384132812158	41.0	41.0	41.0	37.0	41.0
12-13	40.00371143050876	41.0	41.0	41.0	37.0	41.0
14-15	39.97876821406985	41.0	41.0	41.0	37.0	41.0
16-17	39.93434640108097	41.0	41.0	41.0	37.0	41.0
18-19	39.91869974360246	41.0	41.0	41.0	37.0	41.0
20-21	39.909056450175555	41.0	41.0	41.0	37.0	41.0
22-23	39.932066588974905	41.0	41.0	41.0	37.0	41.0
24-25	39.911521183238804	41.0	41.0	41.0	37.0	41.0
26-27	39.87850064025736	41.0	41.0	41.0	37.0	41.0
28-29	39.87319077582596	41.0	41.0	41.0	37.0	41.0
30-31	39.812734849001885	41.0	41.0	41.0	37.0	41.0
32-33	39.857303070534854	41.0	41.0	41.0	37.0	41.0
34-35	39.82746040003319	41.0	41.0	41.0	37.0	41.0
36-37	39.799578272198744	41.0	41.0	41.0	37.0	41.0
38-39	39.78562223293682	41.0	41.0	41.0	37.0	41.0
40-41	39.815464412443575	41.0	41.0	41.0	37.0	41.0
42-43	39.74387790724222	41.0	41.0	41.0	37.0	41.0
44-45	39.5727811878738	41.0	41.0	41.0	37.0	41.0
46-47	39.502378563456915	41.0	41.0	41.0	37.0	41.0
48-49	39.475061540074265	41.0	41.0	41.0	37.0	41.0
50-51	39.466029658056414	41.0	41.0	41.0	37.0	41.0
52-53	39.47595469049891	41.0	41.0	41.0	37.0	41.0
54-55	39.46349689151124	41.0	41.0	41.0	37.0	41.0
56-57	39.43391613610575	41.0	41.0	41.0	37.0	41.0
58-59	39.40050942562925	41.0	41.0	41.0	37.0	41.0
60-61	39.38310371751885	41.0	41.0	41.0	37.0	41.0
62-63	39.35787946786954	41.0	41.0	41.0	37.0	41.0
64-65	39.38704413577777	41.0	41.0	41.0	37.0	41.0
66-67	39.35248067198545	41.0	41.0	41.0	37.0	41.0
68-69	39.278496884146314	41.0	41.0	41.0	37.0	41.0
70-71	39.16601614955901	41.0	41.0	41.0	37.0	41.0
72-73	39.29997766586913	41.0	41.0	41.0	37.0	41.0
74-75	39.29194630872483	41.0	41.0	41.0	37.0	41.0
76-77	38.60095846502226	41.0	39.0	41.0	34.5	41.0
78-79	38.93620466337144	41.0	41.0	41.0	34.5	41.0
80-81	39.24541146846266	41.0	41.0	41.0	37.0	41.0
82-83	39.37168127335602	41.0	41.0	41.0	37.0	41.0
84-85	39.29343067839294	41.0	41.0	41.0	37.0	41.0
86-87	39.26166162005734	41.0	41.0	41.0	37.0	41.0
88-89	39.24633631874604	41.0	41.0	41.0	37.0	41.0
90-91	39.28153206123099	41.0	41.0	41.0	37.0	41.0
92-93	39.236270462826525	41.0	41.0	41.0	37.0	41.0
94-95	39.181844045532905	41.0	41.0	41.0	37.0	41.0
96-97	39.125683955906695	41.0	41.0	41.0	37.0	41.0
98-99	39.075465364973695	41.0	41.0	41.0	37.0	41.0
100-101	38.20361485181037	41.0	39.0	41.0	32.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.046904972263625666
1101	2	-0.21252033306673113
1101	3	-0.5124723271415093
1101	4	-0.24105363177749695
1101	5	-0.3111553174242232
1101	6	-0.4576595772603582
1101	7	-0.4943185034980999
1101	8	-0.49339920972322915
1101	9	-0.5470274846893659
1101	10-11	-0.44655684859930034
1101	12-13	-0.5782515078337269
1101	14-15	-0.5000139418604803
1101	16-17	-0.46271143602940157
1101	18-19	-0.4661271966043543
1101	20-21	-0.5928166989175097
1101	22-23	-0.6067897237414073
1101	24-25	-0.6370882825582456
1101	26-27	-0.6848009615954922
1101	28-29	-0.4684984967406649
1101	30-31	-0.6336710129473389
1101	32-33	-0.8478179330590123
1101	34-35	-0.6869118911017935
1101	36-37	-0.6467445171996715
1101	38-39	-0.6258703553541451
1101	40-41	-0.698065026090255
1101	42-43	-0.8342217602876687
1101	44-45	-0.8094636480373296
1101	46-47	-0.7951419895510838
1101	48-49	-0.8256089984119583
1101	50-51	-0.8967651045142446
1101	52-53	-0.8663626496008803
1101	54-55	-0.9099380990815149
1101	56-57	-0.8974378298841046
1101	58-59	-0.9353146085903603
1101	60-61	-0.876586657583708
1101	62-63	-1.0313582104946022
1101	64-65	-1.089425620246729
1101	66-67	-1.129191299068097
1101	68-69	-0.9563658906292787
1101	70-71	-0.8110643492912928
1101	72-73	-1.01966960983696
1101	74-75	-1.0297957600781658
1101	76-77	-1.39054385502547
1101	78-79	-1.0628987334427649
1101	80-81	-0.8875837560857747
1101	82-83	-1.0158277109607994
1101	84-85	-1.066557331469653
1101	86-87	-0.9727010913227332
1101	88-89	-1.0466628136072131
1101	90-91	-1.0518371128609587
1101	92-93	-1.1598083138864297
1101	94-95	-1.2072598848263496
1101	96-97	-1.2041725607501448
1101	98-99	-1.3862937432857478
1101	100-101	-1.5663725161577773
1102	1	0.09337805406765654
1102	2	-0.04829455041387831
1102	3	0.07902828183379995
1102	4	-0.06038574593369361
1102	5	0.026367404161838692
1102	6	-0.059167527055777214
1102	7	-0.09910342114844894
1102	8	-0.167383039681134
1102	9	-0.16083655161408927
1102	10-11	-0.1144506955569966
1102	12-13	-0.1749226574907894
1102	14-15	-0.14682912521539748
1102	16-17	-0.08613150388139701
1102	18-19	-0.09654130440954134
1102	20-21	-0.09257287882095255
1102	22-23	-0.15307610127666038
1102	24-25	-0.17935086958100044
1102	26-27	-0.14776516727827982
1102	28-29	-0.17669183628027696
1102	30-31	-0.2011687959828663
1102	32-33	-0.20096181405309466
1102	34-35	-0.18446272030170263
1102	36-37	-0.1611217847587696
1102	38-39	-0.14000612556991854
1102	40-41	-0.18613221483212783
1102	42-43	-0.22504943775521014
1102	44-45	-0.2700916239211679
1102	46-47	-0.24039154523828898
1102	48-49	-0.2608472806968578
1102	50-51	-0.33102803742025344
1102	52-53	-0.27859661386452217
1102	54-55	-0.3064437353227305
1102	56-57	-0.2541805295396955
1102	58-59	-0.28648647209158185
1102	60-61	-0.2863838467303239
1102	62-63	-0.255788170055709
1102	64-65	-0.3304401758603319
1102	66-67	-0.25292229388720955
1102	68-69	-0.2879994898756948
1102	70-71	-0.13233069595462865
1102	72-73	-0.29641243367935743
1102	74-75	-0.2605869588212073
1102	76-77	-0.4700883792152126
1102	78-79	-0.36548945420458523
1102	80-81	-0.2091061805434009
1102	82-83	-0.248302169874286
1102	84-85	-0.23258520266861638
1102	86-87	-0.27330415625311133
1102	88-89	-0.23927710226518428
1102	90-91	-0.30685166111535267
1102	92-93	-0.26270251040698156
1102	94-95	-0.27045362912296156
1102	96-97	-0.2171674451736223
1102	98-99	-0.3217598623652549
1102	100-101	-0.2679428813221776
1103	1	0.04530510108539332
1103	2	-0.003268783576245937
1103	3	-0.14812807046538978
1103	4	0.06256051486496261
1103	5	0.03593976403042376
1103	6	0.018755699807620374
1103	7	-0.05172735827100894
1103	8	-0.06623254317924676
1103	9	-0.03368289528616941
1103	10-11	-0.20258424880280046
1103	12-13	-0.1409580360199172
1103	14-15	-0.07484365502323698
1103	16-17	-0.07142867377633166
1103	18-19	-0.07623872964619238
1103	20-21	-0.026913182040168238
1103	22-23	-0.0834166818485258
1103	24-25	-0.04025617488312605
1103	26-27	-0.059566846092820924
1103	28-29	-0.05748187773175317
1103	30-31	-0.03380302949977221
1103	32-33	-0.07619216263849182
1103	34-35	-0.10811188174384512
1103	36-37	-0.09673188120805776
1103	38-39	-0.026722816660367243
1103	40-41	-0.11410076540411751
1103	42-43	-0.11791550079514224
1103	44-45	-0.11752183704323471
1103	46-47	0.00706381797182587
1103	48-49	-0.10388360540110142
1103	50-51	-0.15706365046195714
1103	52-53	-0.15965774815195743
1103	54-55	-0.11881605523184646
1103	56-57	-0.11227900561424775
1103	58-59	-0.15540735235205005
1103	60-61	-0.16686097086023466
1103	62-63	-0.08822167373995171
1103	64-65	-0.2102151010379032
1103	66-67	-0.1200125727318806
1103	68-69	-0.12012460953249615
1103	70-71	-0.04109587094901457
1103	72-73	-0.2809870001819448
1103	74-75	-0.20349827170750956
1103	76-77	-0.08119124750704998
1103	78-79	-0.1905588477661695
1103	80-81	-0.03058388486108754
1103	82-83	-0.14722251648016282
1103	84-85	-0.16529643755639967
1103	86-87	-0.10672204029236809
1103	88-89	-0.08318767874508382
1103	90-91	-0.24067268518557228
1103	92-93	-0.1628526723129866
1103	94-95	-0.1831931458571816
1103	96-97	-0.2174650637815816
1103	98-99	-0.25346696798528967
1103	100-101	-0.09917083344763711
1104	1	-0.019381034565643773
1104	2	-0.03522866072584563
1104	3	0.0818179584013734
1104	4	-0.10854768630703404
1104	5	-0.12513527212111342
1104	6	-0.10570579340399888
1104	7	-0.14058481602313577
1104	8	-0.0978006646210332
1104	9	-0.12859751316617007
1104	10-11	-0.1945683815879775
1104	12-13	-0.12142884365301398
1104	14-15	-0.12736134430338808
1104	16-17	-0.09146872327539057
1104	18-19	-0.10757730085762773
1104	20-21	-0.10173457162245825
1104	22-23	-0.15333504474032367
1104	24-25	-0.13412442752571252
1104	26-27	-0.13537551233407186
1104	28-29	-0.24420199078132754
1104	30-31	-0.15011301636936736
1104	32-33	-0.21172794244610316
1104	34-35	-0.17981849237559544
1104	36-37	-0.18004516569791207
1104	38-39	-0.15082833322499312
1104	40-41	-0.20778629198827048
1104	42-43	-0.16972319364679578
1104	44-45	-0.20261921122204285
1104	46-47	-0.1629564259282006
1104	48-49	-0.23076835726383393
1104	50-51	-0.23408343203465876
1104	52-53	-0.21002750906070133
1104	54-55	-0.2474451555887356
1104	56-57	-0.20352554256164268
1104	58-59	-0.21667551706522659
1104	60-61	-0.180800295197038
1104	62-63	-0.19172500872734588
1104	64-65	-0.2479979436987776
1104	66-67	-0.3404738268048675
1104	68-69	-0.22668888546858312
1104	70-71	-0.14254617286315607
1104	72-73	-0.3560083611317637
1104	74-75	-0.40932957314299045
1104	76-77	-0.5127705919986951
1104	78-79	-0.33658800401980926
1104	80-81	-0.08728296923612788
1104	82-83	-0.15798013891139107
1104	84-85	-0.12317354157400473
1104	86-87	-0.2167335502569756
1104	88-89	-0.23412567235806847
1104	90-91	-0.19569376825589302
1104	92-93	-0.24126052148522348
1104	94-95	-0.26146731046279115
1104	96-97	-0.2841918182804264
1104	98-99	-0.34877571793150963
1104	100-101	-0.3139139583684525
1105	1	0.05187718963506427
1105	2	-0.007099055192583137
1105	3	-0.012336153144133277
1105	4	-0.04087965653935299
1105	5	-0.1759049117465139
1105	6	-0.1576376199643832
1105	7	-0.2077545345758054
1105	8	-0.14836744556683357
1105	9	-0.19760335802671847
1105	10-11	-0.16148018833020217
1105	12-13	-0.15235695584100029
1105	14-15	-0.10168741214828003
1105	16-17	-0.1112860058331151
1105	18-19	-0.0971768730112359
1105	20-21	-0.1302610521568468
1105	22-23	-0.13172849189445657
1105	24-25	-0.10987432353348936
1105	26-27	-0.1600413790075379
1105	28-29	-0.19013305174335215
1105	30-31	-0.15485611314683467
1105	32-33	-0.15382011622230607
1105	34-35	-0.19132031737734678
1105	36-37	-0.13482020930329242
1105	38-39	-0.1313914095458415
1105	40-41	-0.10916689043768457
1105	42-43	-0.21442473926944672
1105	44-45	-0.21957837739578423
1105	46-47	-0.22028429290767804
1105	48-49	-0.21978850864271493
1105	50-51	-0.22240939987542419
1105	52-53	-0.20622548756520587
1105	54-55	-0.25402534104527774
1105	56-57	-0.19402371276636643
1105	58-59	-0.18188279488455095
1105	60-61	-0.2432690596645486
1105	62-63	-0.1938735321775127
1105	64-65	-0.2646641961639489
1105	66-67	-0.22760909540217256
1105	68-69	-0.2120280541502595
1105	70-71	-0.1217461833044311
1105	72-73	-0.24888923544030916
1105	74-75	-0.2122136717157801
1105	76-77	-0.3859220917742121
1105	78-79	-0.2773846909956106
1105	80-81	-0.07850985835621316
1105	82-83	-0.16532099858923033
1105	84-85	-0.1060632441716578
1105	86-87	-0.12062498199275495
1105	88-89	-0.07750233349035085
1105	90-91	-0.19823945770990292
1105	92-93	-0.23863455194380379
1105	94-95	-0.17562314804391832
1105	96-97	-0.28993107921592554
1105	98-99	-0.26046913407942185
1105	100-101	-0.3346338374004105
1106	1	0.029796155984360695
1106	2	0.005396843898296311
1106	3	0.06442266913306582
1106	4	-0.03614993112937981
1106	5	-0.03942304835774735
1106	6	-0.008259078120140373
1106	7	-0.08257962300051958
1106	8	-0.05370869143830248
1106	9	-0.031109662508953306
1106	10-11	-0.1786266334380926
1106	12-13	-0.09973155178834503
1106	14-15	-0.03848030507903388
1106	16-17	-0.058535597350733326
1106	18-19	-0.03999131890400065
1106	20-21	-0.011511978512125154
1106	22-23	-0.10721746128101728
1106	24-25	-0.15373838041985977
1106	26-27	-0.10431650078683674
1106	28-29	-0.0946423516835111
1106	30-31	-0.14982738675583818
1106	32-33	-0.12542031210173832
1106	34-35	-0.10194950667347058
1106	36-37	-0.10611047844056998
1106	38-39	-0.01968670277476292
1106	40-41	-0.10053453268447754
1106	42-43	-0.08386768280698931
1106	44-45	-0.08190464274239417
1106	46-47	-0.1289838869178439
1106	48-49	-0.20150418038861062
1106	50-51	-0.22677546488215938
1106	52-53	-0.23514869448661102
1106	54-55	-0.17918965265568687
1106	56-57	-0.20861409775761075
1106	58-59	-0.2734654849183471
1106	60-61	-0.28148373695261597
1106	62-63	-0.18748350684261084
1106	64-65	-0.2201982568414138
1106	66-67	-0.2075318336177503
1106	68-69	-0.17563940801946387
1106	70-71	-0.10919922462623788
1106	72-73	-0.31359982629606264
1106	74-75	-0.24517054286786788
1106	76-77	-0.3975619441906062
1106	78-79	-0.29683041470655525
1106	80-81	-0.12498889358819554
1106	82-83	-0.17007122826081655
1106	84-85	-0.1602531199774475
1106	86-87	-0.18368613507036713
1106	88-89	-0.1458782593361434
1106	90-91	-0.24015700244250837
1106	92-93	-0.2554694095211545
1106	94-95	-0.2938092984855061
1106	96-97	-0.24925488633671478
1106	98-99	-0.3181064748839333
1106	100-101	-0.21807522323204154
1107	1	0.04032861623117512
1107	2	-0.02927527467201685
1107	3	-0.022582391098268317
1107	4	-0.0891876851131741
1107	5	-0.07283179383183125
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1123	3	-1.0683796681981192
1123	4	-0.7456389849340965
1123	5	-0.40819392702004365
1123	6	-0.33795787418441137
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1123	10-11	-0.15869666749354394
1123	12-13	-0.25841594602189133
1123	14-15	-0.18984323895278266
1123	16-17	-0.19864280787458455
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1123	22-23	-0.149288986499009
1123	24-25	-0.1592084651256016
1123	26-27	-0.08768815605407809
1123	28-29	-0.10062552986374129
1123	30-31	-0.12134217559608373
1123	32-33	-0.13474237546998324
1123	34-35	-0.23264394104430153
1123	36-37	-0.143032902125249
1123	38-39	-0.15338606386341525
1123	40-41	-0.16390344786874778
1123	42-43	-0.2640199358106301
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1123	46-47	-0.29965566636389696
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1123	56-57	-0.37741695883728
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1123	64-65	-0.30491921323296367
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1123	68-69	-0.3322563225115758
1123	70-71	-0.22411132608205975
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1123	74-75	-0.31793917203963673
1123	76-77	-0.4228126600448263
1123	78-79	-0.32551816486964924
1123	80-81	-0.23481524752805427
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1123	84-85	-0.20149201786827575
1123	86-87	-0.27746097810218373
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1123	96-97	-0.22326855385153266
1123	98-99	-0.2647658121075551
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1125	1	-0.2757531265603994
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1125	14-15	-0.23914554233653718
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1125	28-29	-0.22105818465794158
1125	30-31	-0.3363868462559907
1125	32-33	-0.37796348716835126
1125	34-35	-0.3044715655481056
1125	36-37	-0.341506204315408
1125	38-39	-0.3023255884576841
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1125	42-43	-0.3542995345210329
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1125	48-49	-0.40344520826315744
1125	50-51	-0.4393771556590309
1125	52-53	-0.41771499681983215
1125	54-55	-0.4118795923370371
1125	56-57	-0.4360094239065404
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1127	96-97	-0.5224381025633917
1127	98-99	-0.37329179263136325
1127	100-101	-0.36272117774776547
1128	1	-0.45993601097822534
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1128	3	-1.3025462092121032
1128	4	-0.9931424629893613
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1128	6	-0.6464420711816388
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1128	9	-0.4298584432200627
1128	10-11	-0.37041966928019576
1128	12-13	-0.3174701429935922
1128	14-15	-0.2532232446312861
1128	16-17	-0.21584590888303268
1128	18-19	-0.3231339090872325
1128	20-21	-0.2490508482312137
1128	22-23	-0.37158871303660845
1128	24-25	-0.27277401516427346
1128	26-27	-0.2197275149690725
1128	28-29	-0.2875961797815947
1128	30-31	-0.29601800137277223
1128	32-33	-0.3052199036658294
1128	34-35	-0.3673192464580026
1128	36-37	-0.2922251148429922
1128	38-39	-0.29048881691655026
1128	40-41	-0.3197316202884224
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1128	44-45	-0.4043042213022119
1128	46-47	-0.46487119012368794
1128	48-49	-0.5290082584550149
1128	50-51	-0.48512042308762915
1128	52-53	-0.49587074307210344
1128	54-55	-0.4959171083810219
1128	56-57	-0.48297247602207705
1128	58-59	-0.4710436110516838
1128	60-61	-0.5041153147611084
1128	62-63	-0.432674766051349
1128	64-65	-0.4865656703431256
1128	66-67	-0.47109561292295865
1128	68-69	-0.43091089290198425
1128	70-71	-0.27308600709343267
1128	72-73	-0.46773781712298756
1128	74-75	-0.5145148811396396
1128	76-77	-0.4739536918436613
1128	78-79	-0.43842117436522443
1128	80-81	-0.25293601534862376
1128	82-83	-0.33338176164274813
1128	84-85	-0.3799060734832054
1128	86-87	-0.4822295266855008
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1128	90-91	-0.35092214390280674
1128	92-93	-0.450076962973597
1128	94-95	-0.41655256098099613
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1128	98-99	-0.40614791288511753
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1201	1	-0.19501998042743196
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1201	3	0.034245592467620156
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1201	32-33	-0.2039154285933762
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1201	56-57	-0.3915512156137808
1201	58-59	-0.2677046211425207
1201	60-61	-0.32810381930605814
1201	62-63	-0.3233618152915767
1201	64-65	-0.3268648419214486
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1201	68-69	-0.34579599022136875
1201	70-71	-0.2270982711877707
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1201	74-75	-0.4244467996239578
1201	76-77	-0.6640728406810368
1201	78-79	-0.5127855728952326
1201	80-81	-0.3323064651527261
1201	82-83	-0.411806093707213
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1201	88-89	-0.2705539553506142
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1202	3	0.09348228009667281
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1202	7	-0.11443538269870146
1202	8	-0.17680638019595563
1202	9	-0.18007156964893056
1202	10-11	-0.2507888089331587
1202	12-13	-0.2725538155838976
1202	14-15	-0.1869770011082963
1202	16-17	-0.13080099669399914
1202	18-19	-0.21738649476333194
1202	20-21	-0.13095739503608428
1202	22-23	-0.1785878425379437
1202	24-25	-0.18203343636003666
1202	26-27	-0.15190949776998508
1202	28-29	-0.1745459690302411
1202	30-31	-0.16569195482215093
1202	32-33	-0.2281532430447939
1202	34-35	-0.2557681178865323
1202	36-37	-0.25911323867187264
1202	38-39	-0.20758002711121293
1202	40-41	-0.2658589448419164
1202	42-43	-0.27330995801528246
1202	44-45	-0.287571844401846
1202	46-47	-0.3569491485146443
1202	48-49	-0.24333445686609423
1202	50-51	-0.2843979703757853
1202	52-53	-0.31518757255692265
1202	54-55	-0.27672993605503393
1202	56-57	-0.3035615866200274
1202	58-59	-0.28323262549534434
1202	60-61	-0.27150747494724925
1202	62-63	-0.2751035328832927
1202	64-65	-0.24146155057056973
1202	66-67	-0.2895996111351806
1202	68-69	-0.25572483685828473
1202	70-71	-0.21679103147926782
1202	72-73	-0.3390459797662473
1202	74-75	-0.386495530087565
1202	76-77	-0.5018212544150273
1202	78-79	-0.35806396403453533
1202	80-81	-0.2537227568515448
1202	82-83	-0.3474871506376118
1202	84-85	-0.19759796014061237
1202	86-87	-0.2535696088124979
1202	88-89	-0.2266306337310411
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2228	98-99	0.1451976732239828
2228	100-101	0.33426134746709835
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	11.0
14	88.0
15	385.0
16	1518.0
17	3598.0
18	6602.0
19	9960.0
20	13608.0
21	17947.0
22	23488.0
23	29528.0
24	35839.0
25	42800.0
26	51076.0
27	60347.0
28	71573.0
29	84732.0
30	100798.0
31	119515.0
32	144687.0
33	175682.0
34	218811.0
35	277175.0
36	369009.0
37	527698.0
38	899159.0
39	2408293.0
40	1.0599516E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.457355236556506	23.66268007293428	21.320544477951785	28.559420212557434
2	24.616555811504234	23.531614163628557	24.968151701035573	26.88367832383164
3	25.447437522125462	22.057007209278233	23.992288188479197	28.503267080117112
4	25.195569409249657	22.359760806920672	23.922628285922045	28.522041497907622
5	25.930288051982615	22.877281714711337	24.198113253867444	26.994316979438604
6	26.4987319919502	22.74681902363779	23.246116566144465	27.50833241826754
7	26.625911225174683	22.54179716902156	23.85244056383891	26.979851041964842
8	25.688380998300666	23.00687368321303	23.989213332868527	27.315531985617774
9	25.679131881723254	22.764684307459046	24.069724222890084	27.486459587927612
10-11	25.927294208580896	22.971294233500508	23.957291467730467	27.144120090188128
12-13	26.11097646248609	22.869662183614174	23.96362719541617	27.055734158483567
14-15	26.2269287630218	22.834074162816858	23.921090858116713	27.017906216044636
16-17	26.312073417486587	22.799511190019448	23.830511503765194	27.057903888728767
18-19	26.416930100708225	22.695714877523233	23.71962370911591	27.16773131265263
20-21	26.386349747444292	22.76292918343973	23.69437392950216	27.156347139613818
22-23	26.447100759403174	22.787981314043016	23.625379718465915	27.1395382080879
24-25	26.576071916220656	22.757778238638608	23.516172194981447	27.14997765015929
26-27	26.53993187936648	22.82267694351742	23.50901110679581	27.12838007032029
28-29	26.589768400819047	22.813907814943697	23.480889813847085	27.115433970390175
30-31	26.72678995762123	22.8202644737363	23.34003279452234	27.11291277412013
32-33	26.673380928731717	22.82615970857836	23.37822046051781	27.122238902172118
34-35	26.693232764483	22.83080290924303	23.359199354243497	27.116764972030477
36-37	26.75235403665447	22.79210882244433	23.263974878327947	27.19156226257325
38-39	26.71130444581159	22.861609770995063	23.315405677177722	27.111680106015623
40-41	26.764146145811118	22.841943223090077	23.288035546708453	27.10587508439035
42-43	26.824727338252774	22.77216584236805	23.173922425750522	27.229184393628653
44-45	26.80937649630401	22.798015119308605	23.19971774851969	27.192890635867695
46-47	26.84694946927363	22.808038568640075	23.190313412400066	27.15469854968623
48-49	26.891738937025483	22.82915193886524	23.07878909362831	27.200320030480963
50-51	26.84700930091654	22.78567127105325	23.149602834219007	27.217716593811204
52-53	26.856776577583986	22.809574213077315	23.1421999632905	27.1914492460482
54-55	26.955065816697736	22.81191534305541	23.02526276533858	27.207756074908268
56-57	26.92456165326958	22.80267184581098	23.049121477508304	27.223645023411137
58-59	26.8996313919596	22.829334547879196	23.055591896386495	27.21544216377471
60-61	26.965094039056964	22.825066590757594	22.95705128594626	27.25278808423919
62-63	26.931483737782752	22.860069419062334	23.009474276211662	27.19897256694325
64-65	26.933824021539156	22.834837115474382	23.00776088634925	27.223577976637216
66-67	26.995053350361452	22.82983208543914	22.944994699579844	27.23011986461956
68-69	26.969063657150716	22.934234474511435	22.915692904338727	27.181008963999126
70-71	27.024883964602882	22.958173492620034	22.906455928153065	27.11048661462402
72-73	27.05817992180451	22.85278636874974	22.895300862620125	27.193732846825625
74-75	27.028399808440966	22.89487070148753	22.93821852859522	27.138510961476285
76-77	27.0664870238441	22.9153384866335	22.914878178751973	27.103296310770418
78-79	27.125920985795748	22.912341154424134	22.84513690284608	27.11660095693404
80-81	27.10738423445125	22.985346290780527	22.827964476053584	27.079304998714647
82-83	27.10998135486889	22.95608231845179	22.87761914346802	27.0563171832113
84-85	27.142153518692925	23.006552735666638	22.794160384135832	27.057133361504604
86-87	27.15804513883188	23.055653202084123	22.777147060479763	27.00915459860423
88-89	27.184491880719985	23.088411465521826	22.759734302491562	26.967362351266626
90-91	27.260695306222992	23.058005483099567	22.70744197981764	26.9738572308598
92-93	27.265461879420354	23.15264388800664	22.66606367872989	26.91583055384311
94-95	27.293544935237968	23.214715955662406	22.647577702359843	26.84416140673978
96-97	27.379550105400075	23.265793829801385	22.55154632451941	26.80310974027913
98-99	27.451620430494515	23.340197851308083	22.518769507841434	26.689412210355968
100-101	27.550789121451498	23.43151585558903	22.41925026219759	26.598444760761886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20.0
1	30.0
2	39.5
3	52.0
4	77.5
5	99.5
6	123.0
7	146.5
8	174.0
9	216.5
10	278.0
11	337.5
12	397.5
13	487.0
14	596.5
15	767.0
16	1097.0
17	1544.5
18	2323.5
19	3664.5
20	5603.0
21	8471.5
22	12581.5
23	17751.0
24	24426.5
25	32962.5
26	43587.0
27	55944.5
28	69827.5
29	84521.0
30	99667.0
31	116969.5
32	135822.5
33	154532.0
34	174982.5
35	197174.0
36	221289.0
37	248426.5
38	277511.0
39	306878.0
40	332938.5
41	353362.5
42	368299.0
43	380991.0
44	385812.0
45	382439.0
46	377634.5
47	370281.5
48	361753.0
49	355226.0
50	352278.5
51	351511.5
52	350555.5
53	353113.5
54	360765.5
55	374227.5
56	393722.5
57	414041.0
58	431904.5
59	449294.5
60	466350.0
61	481913.5
62	495220.5
63	501514.5
64	500246.5
65	489495.0
66	469670.0
67	444225.5
68	413833.5
69	377711.5
70	335061.0
71	288867.0
72	244611.0
73	203301.0
74	164924.0
75	132797.5
76	107155.0
77	86037.5
78	68752.5
79	54677.5
80	42797.0
81	33540.0
82	26092.0
83	19327.0
84	13848.0
85	9874.5
86	6894.5
87	4828.0
88	3283.5
89	1991.0
90	1179.0
91	661.0
92	375.0
93	234.5
94	152.0
95	99.5
96	71.5
97	58.5
98	46.0
99	47.5
100	84.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11704091116871028
2	0.022855812962363768
3	0.0
4	0.0
5	0.0
6	0.008340775997812126
7	0.0
8	0.0
9	0.0
10-11	0.006502613811392163
12-13	6.751179983512391E-5
14-15	0.0
16-17	0.0
18-19	0.00482709368821136
20-21	0.0012949990695646494
22-23	0.0048178875336883885
24-25	0.0016386955050889168
26-27	0.0
28-29	0.0035566443640413004
30-31	0.0067082179290718575
32-33	0.004704344961238407
34-35	0.0
36-37	0.0016264206323916217
38-39	0.0
40-41	0.005256714232616694
42-43	0.0
44-45	0.0010832575155363064
46-47	0.010841781309886035
48-49	0.0
50-51	0.0
52-53	0.004066051580979054
54-55	0.004750375733853264
56-57	0.004090601326373644
58-59	0.005759984013205799
60-61	0.001663245250483507
62-63	0.004940636260661341
64-65	8.684472433336394E-4
66-67	0.002454974539459051
68-69	0.0020222852768793935
70-71	8.837908342052584E-4
72-73	0.0011108759791052206
74-75	0.0050357665240653784
76-77	3.3755899917561956E-5
78-79	0.003559713082215624
80-81	0.001654039095960536
82-83	0.002789464820460347
84-85	0.00155277139620785
86-87	0.0016601765323091837
88-89	0.002507142748422556
90-91	6.689805620025915E-4
92-93	8.74584679682287E-4
94-95	0.004194937744300653
96-97	0.009061924768778222
98-99	0.0
100-101	0.0024058750486698702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6293448E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.133189123939985
#Duplication Level	Percentage of total
1	37.39815356940981
2	22.625538602801186
3	13.948462498854495
4	8.621475391229705
5	5.493270978430861
6	3.546415352283044
7	2.3974977915028504
8	1.6873936894683756
9	1.0837669185436125
>10	3.1787316896089566
>50	0.019293517867089344
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.137436348647628E-6	6.137436348647628E-6	1.8412309045942885E-5	1.8412309045942885E-5	0.04622717057801393	1.718482177621336E-4
2	6.137436348647628E-6	1.2274872697295256E-5	3.068718174323814E-5	1.8412309045942885E-5	0.053984890122704535	2.5163489029455274E-4
3	1.2274872697295256E-5	1.8412309045942885E-5	6.751179983512391E-5	2.454974539459051E-5	0.06098770499651148	3.436964355242672E-4
4	2.454974539459051E-5	2.454974539459051E-5	1.0433641792700968E-4	2.454974539459051E-5	0.0676775106165374	4.357579807539816E-4
5	3.682461809188577E-5	4.29620544405334E-5	1.1661129062430493E-4	2.454974539459051E-5	0.07453916445432544	5.339569623323436E-4
6	4.29620544405334E-5	4.909949078918102E-5	1.6571078141348596E-4	4.29620544405334E-5	0.0804986151488623	6.321559439107057E-4
7	4.29620544405334E-5	6.137436348647628E-5	2.14810272202667E-4	4.909949078918102E-5	0.08610209453517757	7.058051800944772E-4
8	4.29620544405334E-5	6.137436348647628E-5	2.14810272202667E-4	4.909949078918102E-5	0.09141097697675778	7.91729288975544E-4
9	5.523692713782865E-5	6.751179983512391E-5	2.2708514489996225E-4	4.909949078918102E-5	0.09627796400123534	8.837908342052584E-4
10-11	5.523692713782865E-5	8.899282705539061E-5	2.9766566290941E-4	4.909949078918102E-5	0.10447144152667992	9.819898157836205E-4
12-13	7.671795435809536E-5	1.2581744514727637E-4	4.480328534512769E-4	6.751179983512391E-5	0.1144294320023607	0.0011753190607660209
14-15	9.513026340403824E-5	1.31954881495924E-4	5.124759351120769E-4	6.751179983512391E-5	0.1236539988343781	0.001353304714876802
16-17	1.2274872697295256E-4	1.503671905418669E-4	5.922626076444961E-4	7.364923618377154E-5	0.13212979843186046	0.001503671905418669
18-19	1.6264206323916215E-4	1.687794995878098E-4	6.78186716525563E-4	9.819898157836205E-5	0.14015142773954292	0.0017123447412726884
20-21	2.3629129942293368E-4	1.963979631567241E-4	7.518359527093345E-4	1.1354257244998111E-4	0.14761761905767273	0.0019578421952185935
22-23	2.853907902121147E-4	2.332225812486099E-4	8.193477525444584E-4	1.2274872697295256E-4	0.1545314411044243	0.0020897970767145176
24-25	3.2835284465264813E-4	2.393600175972575E-4	8.899282705539061E-4	1.3502359967024782E-4	0.1611629410791381	0.0022524391399536795
26-27	3.8358977179047673E-4	2.547036084688765E-4	9.75852379434973E-4	1.4729847236754308E-4	0.1677023795086221	0.0024242873577158134
28-29	4.695138806715436E-4	2.9766566290941E-4	0.001006539561178211	1.9026052680807648E-4	0.17444742205578587	0.0026022730118265944
30-31	5.738502985985533E-4	3.191466901296767E-4	0.0010986011064079255	1.963979631567241E-4	0.18141034359332658	0.0028078771295062897
32-33	6.720492801769153E-4	3.5904002639588627E-4	0.0012182811152065542	2.178789903769908E-4	0.1872470455608905	0.002958244320048157
34-35	7.518359527093345E-4	3.8665848996480057E-4	0.0014269539510605735	2.2708514489996223E-4	0.19307761009210572	0.0031699858740764997
36-37	8.408287797647251E-4	4.0813951718506725E-4	0.0015619775507308214	2.4549745394590513E-4	0.19911377874100067	0.003305009473746748
38-39	9.359590431687634E-4	4.234831080566863E-4	0.0016202831960429737	2.6390976299184803E-4	0.20391632268381743	0.0035351633368210338
40-41	0.0010249518702241539	4.2655182623101015E-4	0.0017368944866672789	2.6390976299184803E-4	0.2084580255818167	0.003802141817987206
42-43	0.0010863262337106301	4.3882669892830544E-4	0.0017859939774564598	2.7004719934049565E-4	0.21292607924363216	0.004026158244712844
44-45	0.001135425724499811	4.695138806715436E-4	0.001896467831732117	2.823220720377909E-4	0.21758132471408137	0.004191869026126329
46-47	0.0012336247060781734	5.002010624147817E-4	0.0019823919406131837	2.9152822656076233E-4	0.22235931891150357	0.004329961343970902
48-49	0.0013103426604362685	5.891938894701723E-4	0.0020805909221915456	2.9459694473508616E-4	0.22700535822742982	0.004535565461650597
50-51	0.0013747857420970686	6.137436348647629E-4	0.0022002709309901745	3.007343810837338E-4	0.2318079021702466	0.004777994197422178
52-53	0.0014545724146294878	6.382933802593534E-4	0.002310744785265832	3.13009253781029E-4	0.23717509025713895	0.005026560369542407
54-55	0.001549702678033526	6.536369711309725E-4	0.002356775557880689	3.191466901296767E-4	0.2422323378084246	0.005327294750626141
56-57	0.0016417642232632406	6.659118438282676E-4	0.0024641806939820227	3.2221540830400046E-4	0.24725889817796698	0.0056341665680585224
58-59	0.0017522380775388978	6.965990255715059E-4	0.0025807919846063277	3.4369643552426715E-4	0.2523744513745648	0.005971725567234142
60-61	0.001847368340942936	7.487672345350106E-4	0.002623754039046861	3.713148990931815E-4	0.25727519429895995	0.006204948148482752
62-63	0.0019547734770442695	7.978667253241917E-4	0.0026851284025333376	3.8665848996480057E-4	0.26233551056841986	0.006450445602428656
64-65	0.0020529724586226318	8.162790343701346E-4	0.0027771899477630514	4.0200208083641963E-4	0.2677517981461014	0.006834035374219134
66-67	0.002135827849329375	8.408287797647251E-4	0.002921419701956271	4.1427695353371487E-4	0.2736283934499315	0.007229900018706907
68-69	0.002227889394559089	8.59241088810668E-4	0.0030165499653603094	4.173456717080387E-4	0.2794159959267063	0.007616558508671706
70-71	0.0023322258124860986	8.929969887282299E-4	0.0031055427924157	4.3575798075398155E-4	0.2853999963666377	0.008040041616728394
72-73	0.002494867875725261	9.08340579599849E-4	0.0031423674105075855	4.5723900797424834E-4	0.291089399861834	0.00846352472478508
74-75	0.002869251492992766	9.267528886457918E-4	0.0032098792103427095	4.756513170201912E-4	0.2975981511095748	0.008899282705539061
76-77	0.004375992116585759	9.328903249944395E-4	0.0033111469100953954	4.940636260661341E-4	0.304091558766444	0.009381071458907899
78-79	0.011863664461935864	9.482339158660585E-4	0.003400139737150786	5.278195259836961E-4	0.3103333315330187	0.009939578166634834
80-81	0.038457176160626036	9.819898157836205E-4	0.0035259571822980626	5.431631168553151E-4	0.3176153997606891	0.010495016156187444
82-83	0.0964129876009056	9.973334066552396E-4	0.003614950009353453	5.554379895526104E-4	0.3241487007538245	0.011071935172960321
84-85	0.18588453469149072	0.0010280205883984777	0.0036855305273629005	5.984000439931438E-4	0.33106559151875037	0.01160896085346699
86-87	0.30734133131305297	0.0010771200791876586	0.003746904890849377	6.290872257363818E-4	0.337847458684006	0.012213498333808779
88-89	0.46812988877492356	0.001104738542756573	0.003777592072592615	6.413620984336771E-4	0.3441291247868469	0.012842585559545162
90	0.6183282998172026	0.0011415631608484589	0.003811347972510177	6.505682529566486E-4	0.3488641569298285	0.013330511749262648
>>END_MODULE